Stimultaneous Determination of Ephedrine Alkaloids in Ephedra sinica and Wolbigachul-tang by High Performance Liquid Chromatography (고성능 액체 크로마토그래피를 이용한 마황 및 월비가출탕 전탕액에서 에페드린류의 동시분석)

Mi Young Song (송미영); Jung-Ok Kim (김정옥); Hyun Hee Leem (임현희); Hojun Kim (김호준)

doi:10.15429/jkomor.2020.20.2.97

Stimultaneous Determination of Ephedrine Alkaloids in Ephedra sinica and Wolbigachul-tang by High Performance Liquid Chromatography

Journal of Korean Medicine for Obesity Research
Abbr : J Korean Med Obes Res
2020, 20(2), pp.97-108
DOI : 10.15429/jkomor.2020.20.2.97
Publisher : The Society of Korean Medicine for Obesity Research
Research Area : Medicine and Pharmacy > Korean Medicine
Received : November 6, 2020
Accepted : December 14, 2020
Published : December 30, 2020

Mi Young Song ¹, Jung-Ok Kim ², Hyun Hee Leem ³, Hojun Kim ⁴

¹더리셋한의원
²한국한의학진흥원
³한국한의약진흥원
⁴동국대학교

Accredited

ABSTRACT

Objectives: Ephedra sinica and Wolbigachul-tang which contains Ephedra sinica are used to treat obesity in Korean medicine. The aim of this study was to analyze the quantities of ephedrine alkaloids by high-performance liquid chromatography. Methods: The analysis was performed using a YMC-Triat C18 column with operating at 25℃, and UV detection at 210 nm. The mobile phase used a gradient flow with 0.1% H3PO4 in water and acetonitrile. Specificity, linearity, precision, accuracy, limit of detection, and limit of quantification were measured for validation anaylsis. This method was applied to analyze the quantities of ephedrine alkaloids in Ephedra sinica and Wolbigachul-tang. Results: The concentration per Ephedra sinica (gram) of ephedrine and pseudoephedrine in Ephedra sinica decoction are 4.74±0.22 mg and 2.19±0.10 mg, respectively and in Wolbigachul-tang decoction are 6.39±0.34 mg and 2.97±0.21 mg, respectively. The retention time of ephedrine was 23.6 min and that of pseudoephedrine was 25.8 min, and norephedrine and methylephedrine were not detected. Conclusions: In conclusion, analyzed the concentration of ephedrine alkaloids in Ephedra sinica and Wolbigachul-tang by the developed validation method.

KEYWORDS

Ephedra sinica, Wolbigachul-tang, Ephedrine, Pseudoephedrine, High pressure liquid chromatography

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Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Stimultaneous Determination of Ephedrine Alkaloids in Ephedra sinica and Wolbigachul-tang by High Performance Liquid Chromatography

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* References for papers published after 2023 are currently being built.

Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Stimultaneous Determination of Ephedrine Alkaloids in Ephedra sinica and Wolbigachul-tang by High Performance Liquid Chromatography

ABSTRACT

KEYWORDS

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