Fungi are a major cause of cultural and biological degradation in museum storages and archives libraries. In addition, fungi produce toxins and can cause skin allergies. In particular, fungi coming into contact with the skin through the hands of administrators dealing with cultural properties, archives, and books can lead to secondary infections. To prevent this, applying hand sanitizer, which is readily available in the market, is recommended. However, such hand sanitizer can effectively disinfect against bacteria but not fungi. As such, researching for an alternative is required. In this study, we developed a hand sanitizer composed of active ingredients―geranial, cineol, menthol, and Chamaecyparis obtusa― and performed a disinfection test, which targets 10 kinds of species (Alternaria citrimacularis, Alternaria consortialis, Aspergillus niger, Aspergillus versicolor, Cladosporium sphaerospermum, Coniothyrium aleurites, Penicillium brevicompactum, Penicillium corylophilum, Penicillium paneum, and Penicillium polonicum), of fungi investigated in museums and libraries. It was then confirmed to exhibit excellent disinfecting effects. Thus, this hand sanitizer is expected to prevent skin diseases and secondary infections in administrators dealing with cultural properties, archives, and books.