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The effect of scopoletin on Aβ-induced neuroinflammatory response in microglial BV-2 cells

  • Journal of The Korea Society of Computer and Information
  • Abbr : JKSCI
  • 2020, 25(6), pp.165-170
  • DOI : 10.9708/jksci.2020.25.06.165
  • Publisher : The Korean Society Of Computer And Information
  • Research Area : Engineering > Computer Science
  • Received : May 14, 2020
  • Accepted : June 11, 2020
  • Published : June 30, 2020

Hui-Jin Mun 1,  Hyun-Jeong Cho 1

1건양대학교

Accredited

ABSTRACT

In this paper, it was confirmed that scopoletin inhibits neuroinflammation induced by amyloid beta oligomer (Aβ1-42) in microglial BV-2. The mechanisms of inflammatory cytokines and inflammatory mediators by scopoletin were identified. Alzheimer's disease is the most common neurodegenerative disease, but it is a disease whose specific etiology is unknown, and many studies are trying to solve it. We first measured the cell viability with the CCK-8 assay method to confirm that scopoletin and Aβ1-42 are toxic to BV-2 cells. Expression levels of interleukin 1 beta (IL-1β), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and nuclear factor-κB (NF-κB) in inflammatory reactions induced by Aβ1-42 with western blot were analyzed. The ANOVA assay was used to compare protein expression differences between BV-2 cells treated with Aβ1-42 alone and BV-2 cells pretreated with Aβ1-42 and scopoletin. Therefore, this study suggested that scopoletin is worth developing as a neuroinflammatory protection agent for Alzheimer's disease in the future.

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This paper was written with support from the National Research Foundation of Korea.
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