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Anti-inflammatory Effect of Broccoli Leaf Hexane Fraction in LPS-stimulated RAW264.7 Cells

  • Journal of The Korea Society of Computer and Information
  • Abbr : JKSCI
  • 2022, 27(1), pp.175-181
  • DOI : 10.9708/jksci.2022.27.01.175
  • Publisher : The Korean Society Of Computer And Information
  • Research Area : Engineering > Computer Science
  • Received : December 3, 2021
  • Accepted : January 7, 2022
  • Published : January 28, 2022

Mee-Kyung Kim 1

1서원대학교

Accredited

ABSTRACT

In this study, we tested the anti-inflammatory effects of broccoli leaf hexane fraction to confirm the applicability as a functional material in food and cosmetics. This sample was extracted using 70% ethanol from Broccoli leaf and then fractionated with hexane. The production of pro-inflammatory cytokines (TNF-α, IL-4, IL-6, IL-1β), protein expression of iNOS and COX-2, phosphorylation of MAPKs (ERK, JNK, p38) and NF-κB with broccoli leaf hexane fraction were assayed on LPS-stimulated RAW264.7 cells. The broccoli leaf hexane fraction inhibited the secretion of pro-inflammatory cytokines and protein expression of iNOS and COX-2. Also, the broccoli leaf hexane fraction reduced the phosphorylation of MAPKs and NF-κB. Therefore, it is considered that th broccoli leaf hexane fraction has the potential to be used as a natural anti-inflammatory material in food and cosmetics. In the future, it is considered necessary to study the anti-inflammatory mechanism and identification of major bioactive substances.

Citation status

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