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Inhibitory Effects of Rubus crataegifolius Leaf Water Extract on Adipocyte Differentiation and Adipogenesis in 3T3-L1 Preadipocytes

  • Journal of The Korea Society of Computer and Information
  • Abbr : JKSCI
  • 2024, 29(1), pp.187-194
  • DOI : 10.9708/jksci.2024.29.01.187
  • Publisher : The Korean Society Of Computer And Information
  • Research Area : Engineering > Computer Science
  • Received : December 29, 2023
  • Accepted : January 22, 2024
  • Published : January 31, 2024

Mee-Kyung Kim 1

1서원대학교

Accredited

ABSTRACT

In this study, we examined the effects of Rubus crataegifolius leaf on the inhibition of differentiation and adipogenesis of 3T3-L1 preadipocytes to confirm their potential for use as an anti-obesity functional material. Rubus crataegifolius leaves water extracted using hot water were then concentrated for use, with an extract yield of 4.76%. The result of measuring the rate of 3T3-L1 cell survival of Rubus crataegifolius leaf extract (RCLE) showed growth inhibition of 13% at a concentration of 1,000 μg/mL. Thus, in this study, experiments were performed using RCLE treatment concentrations up to 500 μg/mL. Production of triglycedie in 3T3-L1 cells showed a dose-dependent decrease, and the rate of reduction was 28.7, 40.8, and 51.6% at concentrations of 100, 300, and 500 μg/mL, respectively, compared to the control group. In addition, the results confirmed that suppression of lipogenesis was achieved by suppressing the expression of peroxisome proliferator-activated receptor γ (PPAR γ), CCAAT/ enhancer-binding protein α (C/EBP α), sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), and increasing the expression of p-activated protein kinase (p-AMPK). Based on these results, it is believed that Rubus crataegifolius leaf extract can be used in the effort to manage obesity by regulating factors related to adipocyte differentiation and adipogenesis.

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