Journal of Korean society of Dental Hygiene 2023 KCI Impact Factor : 0.8
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pISSN : 2287-1705 / eISSN : 2288-2294
- https://journal.kci.go.kr/jksdh
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Analysis of total oral microorganisms in saliva using real-time PCR and colony forming unit
- Journal of Korean society of Dental Hygiene
- Abbr : J Korean Soc Dent Hyg
- 2017, 17(1), pp.13-25
- DOI : 10.13065/jksdh.2017.17.01.13
- Publisher : Korean Society of Dental Hygiene
- Research Area : Medicine and Pharmacy > Dentistry
Su-Min Yoo 1, Seong-Kug Jeong 2, 유현준 3, Jong-Hwa Jang 4
1경동대학교
2조선대학교
3단국대학교
4한서대학교
Accredited
ABSTRACT
Objectives: The purpose of this study was to compare colony forming unit (CFU) method and multiplex real-time polymerase chain reaction (MRT-PCR) method for accurate quantitative analysis of bacteria.
Methods: We compared the CFU method and the MRT-PCR method, which are still used in Korea, for Prevotella intermedius (P. intermedius), a periodontal disease pathogen selected by MRT-PCR, and Streptococcus mutans (S. mutans), a dental caries causative organism. The subjects of this study were 30 patients who visited the C dental hospital. Results: Total microorganisms in MRT-PCR method were significantly higher in both types of bacteria (p<0.05), since DNA of dead bacteria was also analyzed. This was because the periodontal dise(-) anaerobes, and even dead bacteria contain large amounts of toxic substances called LPS in the extracellular membrane, and fimbriae and pili, which are motility structures, still remain as a strong toxic substance in periodontal tissue. Conclusions: Therefore, in terms of the total amount of bacteria found, the MRT-PCR method will be a useful technique for searching all the bacteria in the oral cavity including live bacteria, as well as sterilization.
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This paper was written with support from the National Research Foundation of Korea.