Quantitative analysis of oral disease-causing bacteria in saliva among bacterial culture, SYBRgreen qPCR and MRT-PCR method (타액내 구강질환 원인 균의 세균배양법, SYBR green qPCR법, MRT-PCR법 간의 정량분석)

Yong-Duk Park (박용덕); 오혜영; Park bok ri (박복리); A-Ra Cho (조아라); Kim， Dong-Kie (김동기); Jong-Hwa Jang (장종화)

doi:10.13065/jksdh.2017.17.02.319

Quantitative analysis of oral disease-causing bacteria in saliva among bacterial culture, SYBRgreen qPCR and MRT-PCR method

Journal of Korean society of Dental Hygiene
Abbr : J Korean Soc Dent Hyg
2017, 17(2), pp.319-330
DOI : 10.13065/jksdh.2017.17.02.319
Publisher : Korean Society of Dental Hygiene
Research Area : Medicine and Pharmacy > Dentistry

Yong-Duk Park ¹, 오혜영 ¹, Park bok ri ¹, A-Ra Cho ², Kim， Dong-Kie ², Jong-Hwa Jang ³

¹조선대학교 치과대학 예방치과교실
²조선대학교
³한서대학교

Accredited

ABSTRACT

Objectives: The purpose of this study was to compare SYBR Green qPCR, TaqMan, and bacterial selective medium cultures for accurate quantitative analysis of oral microorganisms. Methods: The SYBR Green method is widely used to analyze the total amount of oral microorganisms in oral saliva. However, in this study, MTR-PCR method based on TaqMan method was performed using newly developed primers and probes. In addition, it was designed to confirm the detection agreement of bacteria among bacteria detection method. Results: As a result of MRT-PCR and SYBR Green qPCR analysis, more than 40 times (0.9-362.9 times) bacterium was detected by MRT-PCR. In addition, more bacteria were detected in saliva in the order of MRT-PCR, SYBR Green qPCR, and bacterium culture, and the results of MRB-PCR and SYBR Green qPCR showed the highest agreement. The agreement between the three methods for detecting P. intermedia was similar between 71.4 and 88.6%, but the agreement between MRT-PCR and SYBR Green qPCR was 80% for S. mutans. Among them, the number of total bacteria, P. intermedia and S. mutans bacteria in saliva was higher than that of SYBR Green qPCR method, and bacterium culture method by MRT-PCR method. P. intermedia and S. mutans in saliva were detected by MRT-PCR and MRT-PCR in 88.6% of cases, followed by the SYBR Green qPCR method (80.0%). Conclusions: The SYBR Green qPCR method is the same molecular biology method, but it can not analyze the germs at the same time. Bacterial culturing takes a lot of time if there is no selective culture medium. Therefore, the MRT-PCR method using newly developed primers and probes is considered to be the best method.

KEYWORDS

Bacterial culture, MRT-PCR, Oral microorganisms, Saliva, SYBR Green qPCR

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* References for papers published after 2023 are currently being built.

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This paper was written with support from the National Research Foundation of Korea.

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Journal of Korean society of Dental Hygiene 2023 KCI Impact Factor : 0.8

Quantitative analysis of oral disease-causing bacteria in saliva among bacterial culture, SYBRgreen qPCR and MRT-PCR method

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* References for papers published after 2023 are currently being built.

Journal of Korean society of Dental Hygiene 2023 KCI Impact Factor : 0.8

Quantitative analysis of oral disease-causing bacteria in saliva among bacterial culture, SYBRgreen qPCR and MRT-PCR method

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