@article{ART002401246},
author={김은미},
title={Isolation and identification of the abundant bacteria in dental caries in children},
journal={Journal of Korean society of Dental Hygiene},
issn={2287-1705},
year={2018},
volume={18},
number={5},
pages={843-852},
doi={10.13065/jksdh.20180072}
TY - JOUR
AU - 김은미
TI - Isolation and identification of the abundant bacteria in dental caries in children
JO - Journal of Korean society of Dental Hygiene
PY - 2018
VL - 18
IS - 5
PB - Korean Society of Dental Hygiene
SP - 843
EP - 852
SN - 2287-1705
AB - Objectives: The study aimed to isolate the abundant bacteria in dental caries in children and to investigate the bacterial species involved in addition to those that have been previously reported. Methods: The specimens were collected from the supragingival plaques of each dental caries area, pit and fissure caries, deep dentinal caries, smooth surface caries, and dental caries, and from healthy subjects in the control group. Bacteria were cultured from these specimens, DNA was extracted from the isolated bacteria, and the 16S rRNA gene sequences were analyzed and identified. Results: Based on the results of the 16S rRNA gene sequence analysis for the 90 strains of dominant bacteria from the 45 specimens, 5, 7, 8, 7, and 13 species were identified from the supragingival plaques from healthy teeth, pit and fissure caries, deep dentinal caries, smooth surface caries, and dental caries, respectively. In healthy teeth, Actinomyces naeslundii dominated. Corynebacterium durum, Ralstonia pickettii , and Streptococcus intermedius showed equal distribution. The dominant bacterial species in dental caries, S. sanguinis , showed the greatest difference in prevalence in pit and fissure caries. In deep dentinal caries, S. mutans and Lactobacillus rhamnosus were dominant; in smooth surface caries, S. mutans and S. sanguinis were dominant; and in the supragingival plaques of dental caries, S. sanguinis and S. mutans were dominant. Conclusions: The bacterial species isolated from dental caries encompassed four phyla, eight genera, and 22 species. In addition, the SS1-2 strain, belonging to the genus Neisseria , was identified as a new species from among the isolated strains.
KW - Dental caries;Identification;Isolation;16S rRNA gene
DO - 10.13065/jksdh.20180072
ER -
김은미. (2018). Isolation and identification of the abundant bacteria in dental caries in children. Journal of Korean society of Dental Hygiene, 18(5), 843-852.
김은미. 2018, "Isolation and identification of the abundant bacteria in dental caries in children", Journal of Korean society of Dental Hygiene, vol.18, no.5 pp.843-852. Available from: doi:10.13065/jksdh.20180072
김은미 "Isolation and identification of the abundant bacteria in dental caries in children" Journal of Korean society of Dental Hygiene 18.5 pp.843-852 (2018) : 843.
김은미. Isolation and identification of the abundant bacteria in dental caries in children. 2018; 18(5), 843-852. Available from: doi:10.13065/jksdh.20180072
김은미. "Isolation and identification of the abundant bacteria in dental caries in children" Journal of Korean society of Dental Hygiene 18, no.5 (2018) : 843-852.doi: 10.13065/jksdh.20180072
김은미. Isolation and identification of the abundant bacteria in dental caries in children. Journal of Korean society of Dental Hygiene, 18(5), 843-852. doi: 10.13065/jksdh.20180072
김은미. Isolation and identification of the abundant bacteria in dental caries in children. Journal of Korean society of Dental Hygiene. 2018; 18(5) 843-852. doi: 10.13065/jksdh.20180072
김은미. Isolation and identification of the abundant bacteria in dental caries in children. 2018; 18(5), 843-852. Available from: doi:10.13065/jksdh.20180072
김은미. "Isolation and identification of the abundant bacteria in dental caries in children" Journal of Korean society of Dental Hygiene 18, no.5 (2018) : 843-852.doi: 10.13065/jksdh.20180072
