2017, Vol.2, No.1

Kinesiology KCI Impact Factor : 0.0

pISSN : 2435-0702
https://journal.kci.go.kr/k

The objective of this study was to investigate the protective effect of Cynanchum wilfordii root crude extract (CWR) on acetaminophen(AAP) overdose-induced liver damage. We tested the hepatoprotective effect of CWR in a normal liver cell line(Chang cell) using cell viability assay and western blot analysis. The protective effect of CWR was determined using hematoxylin and eosin staining and immunohistochemistry in vivo. Our results showed that the CWR significantly prevented AAP–induced cell death through the extra cellular signal-regulated kinase 1/2(ERK1/2) activation. AAP-induced liver damage in mice was significantly reduced when CWR was administered at a dose of 100 mg/kg/day(for 7 days) in saline solution. CWR regulated the expressions of inflammation-related proteins such as tumor necrosis factor-α(TNF-α) and inducible nitric oxide synthase(iNOS). These data suggest that CWR presents hepatoprotective effect via the activation of ERK1/2 and the down-regulation of TNF-α and iNOS, which are closely associated with anti-inflammatory responses. Moreover, these findings suggest that CWR can act as a potential therapeutic agent in the treatment of liver damage.

This study was to investigate the effects of band resistance exercise and balance training on the improvement of sports injury and performance in the men's university bowling players. To achieve this purpose, the athletes who have been registered as a member of the Korea Bowling Association with more than 5 years of athletic career were selected, and after observing the enhancement of sports injury and performance through the band resistance exercise and balance training, the following conclusion is obtained. First, As for the Average score, there were significantly increased in score of the athletes in 2014 before the training was 191.26, while the average score of the athletes in 2015 after the training was 196.88, which increased by 5.62 compared to the pre-test. Second, as for the Frequency of Sports Injuries by Period, a total of 196 injuries occurred before training in 2014, of which 86.8% occurred during the preparation season. A total of 94 injuries occurred in 2015 after the training, which was significantly reduced compared to pre-training, yet 76.6% of the injuries occurred during the preparation season. The athletes' injury a rate was reletively higher during the preparation season compared to competition season and break season. Third As for the Sports Injury Type, there were decreased increased in 2015. As for the Sports Injury Type and Sports Injury Area, there were Overall improvement increased in 2015. However, there is a need for more specific exercise programs to be developed for the continual improvement of athletic performance in band resistance exercise and balance training along with further studies to confirm the physiological benefits of those programs.

It has been reported that Cynanchum wilfordii exhibits anti-oxidant, and anti-cancer activities. However, little is known about biological activity of cynanchone A, an acetophenone isolated from C. wilfordii. In the present study, we investigated the anti-inflammatory effect and the underlying molecular mechanisms of cynanchone A in lipopolysaccharide(LPS)-induced RAW 264.7 cells. During the LPS-induced inflammatory process in RAW 264.7 macrophages, nitric oxide(NO) and prostaglandin E2(PGE2) productions increased. Cynanchone A significantly decreased the protein levels of NO and PGE2 in LPS-treated RAW 264.7 macrophages. Moreover, cynanchone A significantly reduced the elevated the expression levels of inducible nitric oxide(iNOS), cyclooxygenase-2(COX-2) and tumor necrosis factor-a(TNF-a) in LPS-stimulated RAW264.7 cells. Consequently, cynanchone A exerted an anti-inflammatory action through inhibiting nuclear factor-kappa B(NF-κB) activation. Taken together, our results show that the anti-inflammatory properties of cyanchone A are involved in the down-regulating pro-inflammatory cytokines and regulating NF-kB signal pathway in LPS-stimulated RAW264.7 cells.