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Development of an In vitro Phototoxicity Test Using the Human Reconstituted Epidermis Model Developed in Korea

  • Journal of Alternatives to Animal Experiments
  • Abbr : J Altern Anim Exp
  • 2016, 10(1), pp.1-9
  • DOI : 10.23032/jaae.2016.10.1.003
  • Publisher : Korean Society for Alternatives to Animal Experiments
  • Research Area : Medicine and Pharmacy > Veterinary > Basic Veterinary Medicine > Laboratory Animal Medicine
  • Received : August 9, 2016
  • Accepted : November 3, 2016
  • Published : November 30, 2016

Yong-Sun Lee 1 Jung-Sun Yi 1 Hye-Rim Lim 2 Tae-Sung Kim 1 Il-Young Ahn 1 Hyung Mo Gu 1 Jae Sung Park 1 Hye-Kyung Park 3 Jong-Kwon Lee 1 Soo-Jung Sohn 4

1식품의약품안전평가원 특수독성과
2식품의약품안전평가원 생약제제과
3식품의약품안전평가원 독성평가연구부
4식품의약품안전평가원 독성연구과

ABSTRACT

Human reconstituted epidermis models have been widely used in evaluating phototoxicity. However, human reconstituted epidermis models made in Korea have not been employed to assess phototoxicity. In this study, we conducted an in vitro phototoxicity test with Neoderm® -E, human reconstituted epidermis model from Korea, in order to see if the model could be used for the evaluation of phototoxicity. Firstly, we performed an UVA sensitivity test and selected 6 J/cm^2 for an UVA irradiation dose. Chlorpromazine as a positive control was then tested to confirm that the substance induces phototoxicity in this model. Afterwards, 3 phototoxins and 2 non-phototoxins were tested using Neoderm® -E and Epiderm TM , a generally used phototoxicity test model, to compare prediction results for each chemical. The results indicated that all those 5 chemicals were correctly predicted with Neoderm® -E, which was accordant with the phototoxic information on them. However, only 3 chemicals were correctly predicted with Epiderm TM . Consequently, the predictive capacity of the test with Neoderm® -E could be higher than that using Epiderm TM . Overall, we reached the conclusion that Neoderm® -E could be used in evaluating phototoxicity.

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