Development of an In vitro Phototoxicity Test Using the Human Reconstituted Epidermis Model Developed in Korea (국내 인체피부모델을 이용한 광독성 동물대체시험법 개발 연구)

Yong-Sun Lee (이용선); Jung-Sun Yi (이정선); Hye-Rim Lim (임혜림); Tae-Sung Kim (김태성); Il-Young Ahn (안일영); Hyung Mo Gu (고경육); Jae Sung Park (김주환); Hye-Kyung Park (박혜경); Jong-Kwon Lee (이종권); Soo-Jung Sohn (손수정)

doi:10.23032/jaae.2016.10.1.003

Development of an In vitro Phototoxicity Test Using the Human Reconstituted Epidermis Model Developed in Korea

Journal of Alternatives to Animal Experiments
Abbr : J Altern Anim Exp
2016, 10(1), pp.1-9
DOI : 10.23032/jaae.2016.10.1.003
Publisher : Korean Society for Alternatives to Animal Experiments
Research Area : Medicine and Pharmacy > Veterinary > Basic Veterinary Medicine > Laboratory Animal Medicine
Received : August 9, 2016
Accepted : November 3, 2016
Published : November 30, 2016

Yong-Sun Lee ¹, Jung-Sun Yi ¹, Hye-Rim Lim ², Tae-Sung Kim ¹, Il-Young Ahn ¹, Hyung Mo Gu ¹, Jae Sung Park ¹, Hye-Kyung Park ³, Jong-Kwon Lee ¹, Soo-Jung Sohn ⁴

¹식품의약품안전평가원 특수독성과
²식품의약품안전평가원 생약제제과
³식품의약품안전평가원 독성평가연구부
⁴식품의약품안전평가원 독성연구과

ABSTRACT

Human reconstituted epidermis models have been widely used in evaluating phototoxicity. However, human reconstituted epidermis models made in Korea have not been employed to assess phototoxicity. In this study, we conducted an in vitro phototoxicity test with Neoderm® -E, human reconstituted epidermis model from Korea, in order to see if the model could be used for the evaluation of phototoxicity. Firstly, we performed an UVA sensitivity test and selected 6 J/cm^2 for an UVA irradiation dose. Chlorpromazine as a positive control was then tested to confirm that the substance induces phototoxicity in this model. Afterwards, 3 phototoxins and 2 non-phototoxins were tested using Neoderm® -E and Epiderm TM , a generally used phototoxicity test model, to compare prediction results for each chemical. The results indicated that all those 5 chemicals were correctly predicted with Neoderm® -E, which was accordant with the phototoxic information on them. However, only 3 chemicals were correctly predicted with Epiderm TM . Consequently, the predictive capacity of the test with Neoderm® -E could be higher than that using Epiderm TM . Overall, we reached the conclusion that Neoderm® -E could be used in evaluating phototoxicity.

KEYWORDS

Human reconstituted epidermis model, phototoxicity, alternative test method, Epiderm TM, Neoderm® -E

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* References for papers published after 2023 are currently being built.

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Journal of Alternatives to Animal Experiments 2023 KCI Impact Factor : 0.07

Development of an In vitro Phototoxicity Test Using the Human Reconstituted Epidermis Model Developed in Korea

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Journal of Alternatives to Animal Experiments 2023 KCI Impact Factor : 0.07

Development of an In vitro Phototoxicity Test Using the Human Reconstituted Epidermis Model Developed in Korea

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