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Comparison of Reconstructed Human Skin Models with Human and Pig Skin in in-vitro Percutaneous Absorption

  • Journal of Alternatives to Animal Experiments
  • Abbr : J Altern Anim Exp
  • 2017, 11(1), pp.1-8
  • DOI : 10.23032/jaae.2017.11.1.003
  • Publisher : Korean Society for Alternatives to Animal Experiments
  • Research Area : Medicine and Pharmacy > Veterinary > Basic Veterinary Medicine > Laboratory Animal Medicine
  • Received : March 7, 2017
  • Accepted : December 19, 2017
  • Published : December 29, 2017

Ju-Hee Han 1 C-Yoon Kim 1 Chae-Hyung Lim 2 Yi-Rang Na 1 Tae-Hyoun Kim 1 Hyun Jung 1 Seung-Hyeok Seok 3 Cho-Rok Jung 4 Jae-Hak Park 1

1서울대학교 수의과대학
2식약처 서울지방식약청
3서울대학교 의과대학
4한국생명공학연구원 줄기세포연구센터

ABSTRACT

This study was carried out in accordance with OECD guidelines on skin absorption test for the comparison of reconstructed human skin models, EFT-306™ and KeraSkinFT™ with human skin and pig skin. Three OECD standard compounds were used for penetration and absorption: Caffeine, benzoic acid and testosterone, all of which differ vastly in their lipophilicity. Reconstructed human skins were more permeable to low lipophilic compounds such as caffeine than human and pig skin. Differences became most obvious with KeraSkinFT™ showing a relatively weak penetration barrier on caffeine. EFT-306™ and KeraSkinFT™ both showed similar penetration patterns in the case of lipophilic compounds, benzoic acid and testosterone compared with human and pig skins. High correlation was observed on the penetration flux between the reconstructed human skin models and human and pig skin, even though different penetration rates existed. The reconstructed human skin models can be regarded as generally useful on lipophilic compounds for in-vitro penetration studies.

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