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Synergistic Hypopigmentation Effects of Mulberroside A and Oxyresveratrol

  • Journal of Alternatives to Animal Experiments
  • Abbr : J Altern Anim Exp
  • 2020, 14(1), pp.59-65
  • DOI : 10.23032/jaae.2020.14.1.007
  • Publisher : Korean Society for Alternatives to Animal Experiments
  • Research Area : Medicine and Pharmacy > Veterinary > Basic Veterinary Medicine > Laboratory Animal Medicine
  • Received : December 9, 2020
  • Accepted : December 24, 2020
  • Published : December 30, 2020

Heui-Jin Park 1 Yoon-Jung Huh 1 Kyung-Min Lim 1

1이화여자대학교 약학대학

Candidate

ABSTRACT

Melasma is an acquired hyper-melanosis characterized by irregular light to dark brown macules and patches on sun-exposed areas of the skin. Topical treatments of melasma involve the use of hypo-pigmenting agents which modifies various stages of melanogenesis. The most common mode of action for hypo-pigmentation is the inhibition of the tyrosinase. Mulberroside A and oxyresveratrol, well known tyrosinase inhibitors, are found frequently together in natural products. The aim of the experiment is to find out the synergism in decreasing pigmentation by mulberroside A and oxyresveratrol. B16F10 murine melanoma cells were used for identification of melanin content and cell viability. In addition, inhibition against tyrosinase, a key enzyme for melanin synthesis, was determined. Single treatment of mulberroside A or oxyresveratrol exhibited dose-dependent inhibition of mushroom tyrosinase activity, which showed a reduction of extra melanin in B16F10 melanoma cells. Interestingly, while mulberroside A or oxyresveratrol rarely reduced melanin inside the melanocytes alone, their mixture could significantly attenuate the production of intracellular melanin, which might be manifested as synergistic hypopigmenting effects. Further studies are needed to understand the mechanism of synergism by mulberroside A and oxyresveratrol in the inhibition of melanin synthesis.

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