Evaluation of Eye Irritation Potential for 30 Chemicals using Two Tiered Approaches

Hye Lyun Jeon (전혜련); Ah Rang Cho (조아랑); Hyung Mo Gu (고경육); Joohwan Kim (김주환); Jae Hoon Jeong (정미경); Kyo-Hyun Park (박교현); Kim, Bae-Hwan (김배환); Won Jun Oh (오원준); Jong Kwon Lee (이종권); Ki Sook Park (박기숙)

doi:10.23032/jaae.2020.14.1.004

Evaluation of Eye Irritation Potential for 30 Chemicals using Two Tiered Approaches

Journal of Alternatives to Animal Experiments
Abbr : J Altern Anim Exp
2020, 14(1), pp.31-45
DOI : 10.23032/jaae.2020.14.1.004
Publisher : Korean Society for Alternatives to Animal Experiments
Research Area : Medicine and Pharmacy > Veterinary > Basic Veterinary Medicine > Laboratory Animal Medicine
Received : August 11, 2020
Accepted : December 14, 2020
Published : December 30, 2020

Hye Lyun Jeon ¹, Ah Rang Cho ¹, Hyung Mo Gu ¹, Joohwan Kim ¹, Jae Hoon Jeong ², Kyo-Hyun Park ², Kim, Bae-Hwan ², Won Jun Oh ³, Jong Kwon Lee ¹, Ki Sook Park ¹

¹식품의약품안전평가원
²계명대학교 자연과학대학 공중보건학과
³한국화학융합시험연구원

Candidate

ABSTRACT

Various alternative test methods are being developed to replace in vivo Draize rabbit test that evaluates eye irritation. However, a single alternative method has difficulty to be applied in safety evaluation on substances, because it cannot fully replace the in vivo test by simulating only part of the in vivo system. For this reason, different studies using several alternative test methods and test results of literatures have been under way. Our previous study suggested effective tiered approaches using three tests among Short Time Exposure (STE), Bovine Corneal Opacity and Permeability (BCOP), Hen’s Egg Test-Chorioallantoic Membrane (HET-CAM), re-constructed human cornea epitheliums (RhCE) tests. This study aimed to evaluate eye irritation potential for 30 new test chemicals using two tiered approaches. According to the data generated by direct test performance and literature survey, the accuracy, sensitivity and specificity of the two tiered approaches were 83.3%∼86.7%, 93.3%, 73.3%∼ 80%, respectively. Furthermore, the accuracy, sensitivity, and specificity of the two tiered approaches by applying combined data against 30 test chemicals in our present study and 47 test chemicals in previous study were 90.9%∼92.2%, 95.9%∼98.0%, 78.6%∼85.7%. Consequently, the two tiered approaches may be used to identify between irritants and non-irritants to replace in vivo test.

KEYWORDS

tiered approach, eye irritation, predictive capacity

Citation status

* References for papers published after 2023 are currently being built.

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Journal of Alternatives to Animal Experiments 2023 KCI Impact Factor : 0.07

Evaluation of Eye Irritation Potential for 30 Chemicals using Two Tiered Approaches

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Journal of Alternatives to Animal Experiments 2023 KCI Impact Factor : 0.07

Evaluation of Eye Irritation Potential for 30 Chemicals using Two Tiered Approaches

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