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Identification of Freshwater Fish Species in Korea Using Environmental DNA Technique – From the Experiment at the Freshwater Fish Ecological Learning Center in Yangpyeong, Gyeonggi Do –

  • Journal of Environmental Impact Assessment
  • Abbr : J EIA
  • 2021, 30(1), pp.1-12
  • Publisher : Korean Society Of Environmental Impact Assessment
  • Research Area : Engineering > Environmental Engineering
  • Received : September 10, 2020
  • Accepted : February 8, 2021
  • Published : February 28, 2021

김가우 1 Song, Youngkeun 2

1국립공원공단 국립공원연구원
2서울대학교 환경대학원 환경조경학과

Accredited

ABSTRACT

This study focused on verifying the identification of freshwater fish species in Korea using Environmental DNA (eDNA) technique. The research of DNA is increasing in the field of ecology, since this is more sensitive of identify rather than traditional investigation method. Which is difficult to detect species hidden in water and be easily influenced by diverse factors (sites, bad weather, researchers and so on). We applied the pilot test in aquarium (Freshwater Fish Ecological Learning Center in Yangpyeong, Gyeonggi Do), where freshwater fish species are inhabits. We conducted to sampling and analyzing the sixteen water samples (50 species from 7 orders and 13 families) using MiFish primer set. The results showed that 45 species (90%) was investigated by eDNA. It highlight that eDNA with universal primer is possible to detect freshwater fish species of Korean. However, the errors on species identification seems to be caused by the primer that be not suited perfectly and the pollution such as aquarium, sampling collectors.

Citation status

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