This study was conducted to identify the bioethical attitude of nurses working in a tertiary hospital. Data were collected from 208 nurses who agreed to participate in the study and analyzed by descriptive statistics, t-test, ANOVA and Scheffé test, and pearson’s correlation. Mean score of bioethical attitude of the subjects was 2.88±0.23 and slightly took up for the position of deontology. Especially, in the areas of right to life fetus, organ transplantation, right to life newborn, artificial insemination, artificial abortion, and euthanasia were more took up for the position of deontology than brain death and human biotechnology. Those who were older,moderate economic level, firm ethical attitudes, the intent to attend class on biomedical ethics, and the possibility of increasing ethical problems according to the development of the life science took up for more deontological position than those who were not. In addition, in the areas of right to life fetus, prenatal diagnosis of fetus, right to life newborn, euthanasia, artificial insemination,organ transplantation, human biotechnology, brain death, and artificial abortion were all significantly correlated each other.