Objectives The purpose of this study is to investigate the effects of korean herbal bathing extracts composition 1 and composition 2 on Th2 cytokine production in MC/9 mast cells.
Methods The effects of composition 1, 2 was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR.
Results Composition 1, 2 inhibited the IL-5, IL-13 production signifcantly(p<.001) in comparison to PI-control group at concentration of 100 ㎍/mL, 200 ㎍/mL. Composition 1, 2 inhibited the IL-4, IL-5, IL-13 mRNA expression signifcantly in comparison to PI-control group at concentration of 100 ㎍/mL, 200 ㎍/mL. Composition 1 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 200 ㎍/㎖. Composition 2 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/mL, 200 ㎍/mL.
Conclusions These results indicate that composition 1, 2 has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.