@article{ART001179437},
author={황영명 and Young Beob Yu and Koh Byung-Hee and Eui Ju Lee and 김종열},
title={HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang},
journal={Journal of Sasang Constitution and Immune Medicine},
issn={1226-4075},
year={2006},
volume={18},
number={1},
pages={147-154}
TY - JOUR
AU - 황영명
AU - Young Beob Yu
AU - Koh Byung-Hee
AU - Eui Ju Lee
AU - 김종열
TI - HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang
JO - Journal of Sasang Constitution and Immune Medicine
PY - 2006
VL - 18
IS - 1
PB - The Society of Sasang Constitution and Immune Medicine
SP - 147
EP - 154
SN - 1226-4075
AB - HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang
Hwang Young-Myung, Yu Young-Beob*, Kim Jong-Youl*, Koh Byung-Hee, Lee Eui-Ju
*Dept. of Medical Research, Korea Institute of Oriental Medicine
Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Kyung-Hee Univ.
1. Objectives
This study was aimed to evaluate marker substances in Gamijowi-tang by high performance liquid chromatography (HPLC)
2. Methods
The seperation was performed an AquasilC18(4.6X250mm)column by gradient elution with 0.1N H3PO4 in H2O - acetonitrile(0min 100:10,20min60:40, 40min 80:20, 60min 100:0) as the mobile phase at a flow-rate of 1.0 ml/min with detection at 190-800nm
3. Results
Ephedrine and schizandrin were established as marker substances in Gamijowi-tang and its qualitative analysis was carried out. Analyzed was similarity of marker substances between in standard article and in Gamijowi-tang. The result of the analysis was that ephedrine and schizadrin showed 0.9999 of similarity, which meant they were really ephedrine and schizadrin. In addition, ephedrin content was 12.16mg, pseudoephedrine content was 5.09mg, and schizandrin content was 26.6mg in 76g of Gamijowi-tang.
4. Conclusions
Qualitative and quantitative analyses of ephedrine and schizandrin in Gamijowi-tang were conducted, and it was identified that they could be used as marker substances in standardizing quality of Gamijowi-tang.
KW - Gamijowi-tang;HPLC;Marker substances;Ephedrin;Schizandrin
DO -
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황영명, Young Beob Yu, Koh Byung-Hee, Eui Ju Lee and 김종열. (2006). HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang. Journal of Sasang Constitution and Immune Medicine, 18(1), 147-154.
황영명, Young Beob Yu, Koh Byung-Hee, Eui Ju Lee and 김종열. 2006, "HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang", Journal of Sasang Constitution and Immune Medicine, vol.18, no.1 pp.147-154.
황영명, Young Beob Yu, Koh Byung-Hee, Eui Ju Lee, 김종열 "HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang" Journal of Sasang Constitution and Immune Medicine 18.1 pp.147-154 (2006) : 147.
황영명, Young Beob Yu, Koh Byung-Hee, Eui Ju Lee, 김종열. HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang. 2006; 18(1), 147-154.
황영명, Young Beob Yu, Koh Byung-Hee, Eui Ju Lee and 김종열. "HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang" Journal of Sasang Constitution and Immune Medicine 18, no.1 (2006) : 147-154.
황영명; Young Beob Yu; Koh Byung-Hee; Eui Ju Lee; 김종열. HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang. Journal of Sasang Constitution and Immune Medicine, 18(1), 147-154.
황영명; Young Beob Yu; Koh Byung-Hee; Eui Ju Lee; 김종열. HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang. Journal of Sasang Constitution and Immune Medicine. 2006; 18(1) 147-154.
황영명, Young Beob Yu, Koh Byung-Hee, Eui Ju Lee, 김종열. HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang. 2006; 18(1), 147-154.
황영명, Young Beob Yu, Koh Byung-Hee, Eui Ju Lee and 김종열. "HPLC Analysis of Marker Substances in Taeumin Gamijowi-tang" Journal of Sasang Constitution and Immune Medicine 18, no.1 (2006) : 147-154.