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Effects Of TAM (Taraxacum mongolicum) On Th2 Cytokine Production In MC/9 Mast Cells

  • Journal of Sasang Constitution and Immune Medicine
  • Abbr : J Sasang Constitut Med
  • 2012, 24(1), pp.54-65
  • Publisher : The Society of Sasang Constitution and Immune Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

장문희 1 최재송 1 Nayoung, Bae 1 TaekWon ahn 1

1대전대학교

Accredited

ABSTRACT

1. Objectives The purpose of this study is to investigate the effects of TAM (Taraxacum mongolicum) on Th2 cytokine production in MC/9 mast cells. 2. Methods The effects of TAM was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. 3. Results 1) TAM inhibited the IL-4 production significantly in comparison to PI-control group at concentration of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖. 2) TAM inhibited the IL-13 production significantly in comparison to PI-control group at concentration of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖. 3) TAM inhibited the IL-4 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/㎖. 4) TAM inhibited the IL-5 mRNA expression significantly in comparison to PI-control group at concentration of 50 ㎍/㎖, 100 ㎍/㎖. 5) TAM inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/㎖. 6) TAM inhibited the IL-13 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/㎖. 4. Conclusion These results indicate that TAM (Taraxacum mongolicum) has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.

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