Effects of Yeoldahanso-tang, a Sasang Constitutional Herbal Prescription for Taeeumin, on the Adipogenesis in 3T3-L1 Cells (太陰人 熱多寒少湯이 3T3-L1 脂肪前驅細胞 分化에 미치는 影響)

Sae-Rom Yoo (유새롬); Soo-Jin Jeong (정수진); 신현규

doi:10.7730/JSCM.2012.24.4.75

Effects of Yeoldahanso-tang, a Sasang Constitutional Herbal Prescription for Taeeumin, on the Adipogenesis in 3T3-L1 Cells

Journal of Sasang Constitution and Immune Medicine
Abbr : J Sasang Constitut Med
2012, 24(4), pp.75-83
DOI : 10.7730/JSCM.2012.24.4.75
Publisher : The Society of Sasang Constitution and Immune Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

Sae-Rom Yoo ¹, Soo-Jin Jeong ¹, 신현규 ²

¹한국한의학연구원
²한국한의학연구원 한약기초연구그룹

Accredited

ABSTRACT

Objectives Although Yeoldahanso-tang (YDHST) has been widely used for treatment of obesity and its related diseases such as hyperinsulinemia and hypertension for Taeeumin, no scientific evidence has reported yet to support its ability to work against these metabolic disorders. Our study was aimed to investigate the anti-obesity effect of YDHST extract on the cellular differentiation of 3T3-L1 preadipocytes into adipocytes. Methods 3T3-L1 preadipocytes were differentiated into adipocytes by adding insulin, dexamethasone and 3-isobutyl-1-methylxanthine (IBMX) for 8 days in the absence or presence of YDHST extract. Anti-obesity effects of YDHST extract were evaluated by Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, triglyceride contents, and leptin production. Results YDHST extract remarkably prevented lipid accumulation with no cytotoxicity in the differentiated 3T3-L1 cells. In addition, YDHST extract decreased contents of triglyceride 3T3-L1 adipocytes. Consistently, YDHST extract caused a significant inhibition of GPDH activity and leptin production in a dose-dependent manner. Conclusions Our findings suggest that Sasang constitutional herbal formula YDHST for Tae-eumin has anti-obesity activity by regulation of the adipogenesis process in vitro. Additional study will be required to further confirm the inhibitory effect on adipocyte differentiation by using in vivo animal model.

KEYWORDS

Yeoldahanso-tang, Taeeumin, 3T3-L1, adipogenesis, anti-obesity

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Effects of Yeoldahanso-tang, a Sasang Constitutional Herbal Prescription for Taeeumin, on the Adipogenesis in 3T3-L1 Cells

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Journal of Sasang Constitution and Immune Medicine 2023 KCI Impact Factor : 0.42

Effects of Yeoldahanso-tang, a Sasang Constitutional Herbal Prescription for Taeeumin, on the Adipogenesis in 3T3-L1 Cells

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