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Neuroprotective Effects of Hyangsayangwi-tang in MPTP-inducedMouse Model of Parkinson's Disease

  • Journal of Sasang Constitutional Medicine
  • Abbr : J Sasang Constitut Med
  • 2014, 26(2), pp.165-179
  • DOI : 10.7730/JSCM.2014.26.2.
  • Publisher : The Society Of Sasang Constitutional Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

고가연 1 김윤희 2 TaekWon ahn 3

1대전대학교 한의과대학 사상체질의학과교실
2대전대학교 한의과대학 사상체질의학
3대전대학교

Accredited

ABSTRACT

ObjectivesTo evaluate the neuroprotective effects of Hyangsayangwi-tang (HY), a Korean traditional medicinal prescription in a Parkinson's disease mouse model. MethodsFour groups(each of 10 mouse per group) were used in this study. The neuroprotective effect of HY was examined in a Parkinson's disease mouse model. C57BL/6 mouse treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30mg/kg/day), intraperitoneal (i.p.) for 5 days. Slow behavioral responses and memory disorder is the major clinical symptoms of PD. In order to investigate the effect of HY on recovery of behavioral deficits and memory, we examined the motor function and memory by using Morris water maze and Forced swimming test. Ischemic mouse brain stained with TTC(2,3,5 triphenyl tetrazolium chloride) in the MPTP-induced Parkinson's disease to find out ischemia and tissue damage in mouse. The convenient, simple, and accurate high-performance liquid chromatography (HPLC) method was established for simultaneous determination of neurotransmitters in MPTP-HY group. To measure the amount of dopamine in mice brain, striatum-substantia nigra, was examined by Bradford assay. Immunohistochemistry was examined in the MPTP-induced Parkinson's disease (PD) mouse to evaluate the neuroprotective effects of Hyangsayangwi-tang on hippocampal lesion, ST and SNpc. Results and ConclusionsHyangsayangwi-tang (HY) prevents MPTP-induced loss of serotonin, hippocampus and TH-ir cell.

Citation status

* References for papers published after 2022 are currently being built.

This paper was written with support from the National Research Foundation of Korea.