A Study of the Effect on Obesity in Taeeumin Animal - experimental Model Induced Lung Fibrosis with Bleomycin (Bleomycin으로 유발한 폐약(肺弱) 태음인 동물 모델에서의 비만에 대한 영향 평가)

김윤하; PARK JUNGHWAN (박정환); jinyoung kwak (곽진영); Jung-Mi Park (박정미); TaekWon ahn (안택원)

doi:10.7730/JSCM.2016.28.2.147

A Study of the Effect on Obesity in Taeeumin Animal - experimental Model Induced Lung Fibrosis with Bleomycin

Journal of Sasang Constitution and Immune Medicine
Abbr : J Sasang Constitut Med
2016, 28(2), pp.147-162
DOI : 10.7730/JSCM.2016.28.2.147
Publisher : The Society of Sasang Constitution and Immune Medicine
Research Area : Medicine and Pharmacy > Korean Medicine
Published : June 30, 2016

김윤하 ¹, PARK JUNGHWAN ¹, jinyoung kwak ¹, Jung-Mi Park ², TaekWon ahn ²

¹대전대학교부속한방병원
²대전대학교

Accredited

ABSTRACT

Objectives The objective of this study is to develop a taeeumin animal-experimental model induced lung fibrosis with Bleomycin and evaluate the effect on obesity in this animal-experimental model. Methods The subjects were divided into 3 groups : normal group, high fat diet(HFD) control group, and HFD group administered with bleomycin(n=10 per group). To develop taeeumin animal-experimental model with reduced respiratory metabolism, 8-week-old C57BL/6 mice were administered with 0.03ml solution of bleomycin 1U/ml dissolved in distilled water, intratracheal(IT), once. Then, the HFD control group and the experimental group were fed with high fat diet for 6 weeks. Airway hyperresponsiveness(AHR) to methacholine was measured at the 1st and 3rd week after bleomycin was administered. Food intake and body weight were measured at regular time weekly. After the final experiment, blood was gathered by cardiac puncture for bloodchemical examination and organs(liver, fatty tissue) were remoed, weighted, and mRNA was analyzed. Results and Conclusions Through the experiment, it was found that Bleomycin induced Taeeumin animal-experimental models have leptin resistace. In the experimental group administered with Bleomycin, fatty acid synthesizing gene expression increased and energy metabolizing gene expression decreased. As mRNA expression of adiponectin decreased, it was found that Taeeuim animal-experimental model is susceptible to metabolic syndrome and cardiovascular diseases.

KEYWORDS

Taeeumin, Obesity, Leptin, Adiponectin, Bleomycin, Animal-experimental model

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Journal of Sasang Constitution and Immune Medicine 2023 KCI Impact Factor : 0.42

A Study of the Effect on Obesity in Taeeumin Animal - experimental Model Induced Lung Fibrosis with Bleomycin

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* References for papers published after 2023 are currently being built.

Journal of Sasang Constitution and Immune Medicine 2023 KCI Impact Factor : 0.42

A Study of the Effect on Obesity in Taeeumin Animal - experimental Model Induced Lung Fibrosis with Bleomycin

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