@article{ART002743408},
author={Soo-jung Park and Kim Hyoung-soon and Young Chun Bae and Lee Sang min and Kim Kyung yo and Kyoung Sook Won and Shim Gyue hearn},
title={Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells},
journal={Journal of Sasang Constitutional Medicine},
issn={1226-4075},
year={2003},
volume={15},
number={1},
pages={72-89}
TY - JOUR
AU - Soo-jung Park
AU - Kim Hyoung-soon
AU - Young Chun Bae
AU - Lee Sang min
AU - Kim Kyung yo
AU - Kyoung Sook Won
AU - Shim Gyue hearn
TI - Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells
JO - Journal of Sasang Constitutional Medicine
PY - 2003
VL - 15
IS - 1
PB - The Society Of Sasang Constitutional Medicine
SP - 72
EP - 89
SN - 1226-4075
AB - To elucidate the neuroprotective effect of Yeoldahanso-tang(YHT) on nerve cells damaged by hypoxia, the cytotoxic effects of exposure to hypoxia were determined by XTT(SODIUM3,3'-{I-[(PHENYLAMINO) CARBONYL]-3,4-TETRAZOLIUM}- BIS (4-METHOXY-6-NITRO) BENZENE SULFONIC ACID HYDRATE), NR(Neutral red), MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and SRB(Sulforhodamin B) asssay. The activity of catalase and SOD(Superoxide dismutase) was measured by spectrophometry, and TNF−α(Tumor cell necrosis fector−α) and PKC(Protein kinase C) activity was measured after exposure to hypoxia and treatment of YHTWE. Also the neuroprotective effect of YHTWE was researched for the elucidatioion of neuroprotective mechanism. The results were as follows; 1. Hypoxia decreased cell viability measured by XTT, NR assay when cultured cerebral neurons were exposed to 95% N2/5% CO2 for 2∼26 minutes in these cultures and YHTWE inhibited the decrease of cell viability. 2. H2O2 treatment decreased cell viability measured by MTT, and SRB assay when cultured cerebral neurons were exposed to 1-80 μM for 6 hours, but YHTWE inhibited the decrease of cell viability. 3. Hypoxia decreased catalase and SOD activity, and also TNF−α and PKC activity in these cultured cerebral neurons, but YHTWE inhibited the decrease of the catalase and SOD activity in these cultures. 4. Hypoxia triggered the apoptosis via caspase activation and internucleosomal DNA fragmentation. Also hypoxia stimulate the release of cytochrome c forom mitochondria. YHTWE inhibited the apoptosis via caspase activation induced by hypoxia. From these results, it can be suggested that brain ischemia model induced hypoxia showed neurotoxicity on cultured mouse cerebral neurons, and the YHTWE has the neuroprotective effect in blocking the neurotoxicity induced by hypoxia in cultured mouse cerebral neurons.
KW - Yeoldahanso-tang;Hypoxic Damage
DO -
UR -
ER -
Soo-jung Park, Kim Hyoung-soon, Young Chun Bae, Lee Sang min, Kim Kyung yo, Kyoung Sook Won and Shim Gyue hearn. (2003). Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells. Journal of Sasang Constitutional Medicine, 15(1), 72-89.
Soo-jung Park, Kim Hyoung-soon, Young Chun Bae, Lee Sang min, Kim Kyung yo, Kyoung Sook Won and Shim Gyue hearn. 2003, "Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells", Journal of Sasang Constitutional Medicine, vol.15, no.1 pp.72-89.
Soo-jung Park, Kim Hyoung-soon, Young Chun Bae, Lee Sang min, Kim Kyung yo, Kyoung Sook Won, Shim Gyue hearn "Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells" Journal of Sasang Constitutional Medicine 15.1 pp.72-89 (2003) : 72.
Soo-jung Park, Kim Hyoung-soon, Young Chun Bae, Lee Sang min, Kim Kyung yo, Kyoung Sook Won, Shim Gyue hearn. Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells. 2003; 15(1), 72-89.
Soo-jung Park, Kim Hyoung-soon, Young Chun Bae, Lee Sang min, Kim Kyung yo, Kyoung Sook Won and Shim Gyue hearn. "Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells" Journal of Sasang Constitutional Medicine 15, no.1 (2003) : 72-89.
Soo-jung Park; Kim Hyoung-soon; Young Chun Bae; Lee Sang min; Kim Kyung yo; Kyoung Sook Won; Shim Gyue hearn. Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells. Journal of Sasang Constitutional Medicine, 15(1), 72-89.
Soo-jung Park; Kim Hyoung-soon; Young Chun Bae; Lee Sang min; Kim Kyung yo; Kyoung Sook Won; Shim Gyue hearn. Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells. Journal of Sasang Constitutional Medicine. 2003; 15(1) 72-89.
Soo-jung Park, Kim Hyoung-soon, Young Chun Bae, Lee Sang min, Kim Kyung yo, Kyoung Sook Won, Shim Gyue hearn. Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells. 2003; 15(1), 72-89.
Soo-jung Park, Kim Hyoung-soon, Young Chun Bae, Lee Sang min, Kim Kyung yo, Kyoung Sook Won and Shim Gyue hearn. "Influence of Yeoldahanso-tang on the Hypoxic Damage of Cultured Cerebral Neurons from mouse and SK-N-MC cells" Journal of Sasang Constitutional Medicine 15, no.1 (2003) : 72-89.
