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Effects of Hyungbangjihwangtang Water Extract on Cultured Primary hippocampal Cell Damaged by Glucose Oxidase

  • Journal of Sasang Constitution and Immune Medicine
  • Abbr : J Sasang Constitut Med
  • 2003, 15(2), pp.137-150
  • Publisher : The Society of Sasang Constitution and Immune Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine
  • Received : August 31, 2003
  • Accepted : August 31, 2003
  • Published : August 30, 2003

Choi Yong Seok 1 Kim Kyung yo 2 Young Chun Bae 3 Noh Hyun-soo 4 Kim Jong-kwan 4 Han Byung-sam 5 Gwon Duk Yun 1

1원광대학교
2원광대학교 한의과대학 사상체질의학교실
3원광대학교 한의과대학 사상체질과
4원광대학교 한의과대학 사상의학교실
5원광대학교 대학원 사상의학교실

ABSTRACT

The purpose of this study is to examine the toxic effects caused by glucose oxidase (GO) and the effects of Hyungbangjihwangtang (HJT) water extracts on the treatment of the toxic effects. For this purpose, experiments with the cultured hippocampal cells from new born mice were done. The results of these experiments were as follows. 1. GO decreased the survival rate of the cultured cells on MTT assay and NR assay. 2. HJT has the efficacy of decreasing the lipid peroxidation. 3. HJT has the efficacy of increasing the amount of neurofilaments. 4. HJT has the efficacy of decreasing the activation of protein kinase C(PKC). From the above results, it is concluded that HJT has marked efficacy as a treatment for the damages caused by the GO-mediated oxidative stress. Further clinical study of this pharmacological effects of H]T should be completed.

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