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Genetic Analysis of Sasang constitution by Amp-FLP Method

  • Journal of Sasang Constitution and Immune Medicine
  • Abbr : J Sasang Constitut Med
  • 1997, 9(2), pp.163-173
  • Publisher : The Society of Sasang Constitution and Immune Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine
  • Received : December 30, 1997
  • Accepted : December 30, 1997
  • Published : December 30, 1997

Dong Wuk Cho 1 Sun Kyung Ahn 1 Do Kyun Ki 1 Dae Won Kim 1 Sang En Ji 1 Eui Ju Lee 2 Hong, Seok-Cheol 3 Koh Byung-Hee 4 Whang Sung Cho 1

1
2경희대학교
3경희대학교 한의과대학 사상의학교실
4경희대학교 한의과대학 사상체질과

ABSTRACT

Amp-FLP is a one of the most frequently used human genetic analysis methos which adopts STR and VNTR typings. In this study, 100 genomic DNA samples of Taeum, Soyang, and Soum constitution group were analysed by Amp-FLP method. The allele distribution of three STR(THO1, vWA and CSF1PO) and on VNTR locus(MCT118) of each different constitution group were investigated and the allele distribution was statistically evaluated. It was found out that thte allele distribution of MCT118 and THO1 loci was not significantly different among different constitutions. However, the allele distribution of vWA showed p-value of 0.02056(Soyang group) and 2.41X10^-10(Soun group) which is much lower than significant level of p-value 0.05. Also, p-value of CSF1PO in Soum group was found out to be 4.65X10^-17. Therefore, it is expected that vWA and CSF1PO loci can be used as an indicator for gnenetic difference of different constitution if the same result is obtained with sufficient number of samples.

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