@article{ART001190913},
author={박범준 and Chung Yoon Hee and 김성수 and 김경용 and 이원복},
title={Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2007},
volume={20},
number={2},
pages={145-155},
doi={10.11637/aba.2007.20.2.145}
TY - JOUR
AU - 박범준
AU - Chung Yoon Hee
AU - 김성수
AU - 김경용
AU - 이원복
TI - Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum
JO - Anatomy & Biological Anthropology
PY - 2007
VL - 20
IS - 2
PB - 대한체질인류학회
SP - 145
EP - 155
SN - 2671-5651
AB - In the present study, we investigated the expression of apoptosis-associated proteins in the cerebellum of aged rats:
IGF-I receptor (IGF-IR), nitrotyrosine (NT), p53, key pro-apoptotic gene ICH-1 (caspase-2), c-Fos and Bcl-2 family
members (Bcl-2 and Bax).
Twelve adult (4~6 month old) and 15 aged (24~29 month old) Sprague-Dawley rats were examined in this study.
We performed immunohistochemical staining, in situ hybridization and densitometric measurement using a NIH image
program (Scion Image) to determine the staining density.
In adult rats, there were no immunoreactivities for insulin-like growth factor-I receptor (IGF-IR), nitrotyrosine (NT)
or p53 in any region of cerebellum. However, IGF-IR immunoreactivity was found in some Purkinje cells in aged rat
cerebellum. The prominent staining of NT or p53 was also localized in the Purkinje cell layer in aged rats. A high density
of ICH-1 (caspase-2) immunoreactivity was observed in the molecular and Purkinje cell layers in aged rats. Immunoreactivity
for c-Fos was significantly decreased in the granule cells in aged rats. Positive signal for bcl-2 was significantly
decreased in the Purkinje cells and granule cells of aged rats. The most intense staining for Bax was observed in
the soma of Purkinje cells of adult rats. However, Bax immunoreactivity was not changed in any layers in the cerebellar
cortex of aged rats.
In conclusion, this study provides the first morphological data concerning the differential regulation of apoptosisrelated
genes in rat cerebellum during aging.
KW - Apoptosis-associated factors;Aging;Cerebellum;Immunohistochemistry;In situ hybridization
DO - 10.11637/aba.2007.20.2.145
ER -
박범준, Chung Yoon Hee, 김성수, 김경용 and 이원복. (2007). Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum. Anatomy & Biological Anthropology, 20(2), 145-155.
박범준, Chung Yoon Hee, 김성수, 김경용 and 이원복. 2007, "Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum", Anatomy & Biological Anthropology, vol.20, no.2 pp.145-155. Available from: doi:10.11637/aba.2007.20.2.145
박범준, Chung Yoon Hee, 김성수, 김경용, 이원복 "Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum" Anatomy & Biological Anthropology 20.2 pp.145-155 (2007) : 145.
박범준, Chung Yoon Hee, 김성수, 김경용, 이원복. Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum. 2007; 20(2), 145-155. Available from: doi:10.11637/aba.2007.20.2.145
박범준, Chung Yoon Hee, 김성수, 김경용 and 이원복. "Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum" Anatomy & Biological Anthropology 20, no.2 (2007) : 145-155.doi: 10.11637/aba.2007.20.2.145
박범준; Chung Yoon Hee; 김성수; 김경용; 이원복. Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum. Anatomy & Biological Anthropology, 20(2), 145-155. doi: 10.11637/aba.2007.20.2.145
박범준; Chung Yoon Hee; 김성수; 김경용; 이원복. Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum. Anatomy & Biological Anthropology. 2007; 20(2) 145-155. doi: 10.11637/aba.2007.20.2.145
박범준, Chung Yoon Hee, 김성수, 김경용, 이원복. Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum. 2007; 20(2), 145-155. Available from: doi:10.11637/aba.2007.20.2.145
박범준, Chung Yoon Hee, 김성수, 김경용 and 이원복. "Differential Expression of Neuronal Death-related Factors in Aged Rat Cerebellum" Anatomy & Biological Anthropology 20, no.2 (2007) : 145-155.doi: 10.11637/aba.2007.20.2.145
