Expressions of Nitric Oxide Synthases and Akt in the Tibialis Anterior Muscle of the Spontaneously Hypertensive Rat after Ischemic Preconditioning (본태성 고혈압 흰쥐의 앞정강근에서 허혈양상화 후 산화질소합성효소와 Akt의 발현 )

최성욱; 서윤경; Paik Doo Jin (백두진); 김태승

doi:10.11637/aba.2008.21.4.317

Expressions of Nitric Oxide Synthases and Akt in the Tibialis Anterior Muscle of the Spontaneously Hypertensive Rat after Ischemic Preconditioning

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2008, 21(4), pp.317~329
DOI : 10.11637/aba.2008.21.4.317
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

최성욱 ¹, 서윤경 ¹, Paik Doo Jin ¹, 김태승 ¹

¹한양대학교

Accredited

ABSTRACT

Nitric oxide (NO) generated during ischemic-reperfusion phase is known to activate various transcriptional factors. NO is generated by 3 nitric oxide synthase (NOS) isoforms, nNOS, iNOS, eNOS. Akt plays a important role of the cell survival. Ischemic preconditioning (IP) reduces ischemic injury by changing of the expression of the NOSs and Akt. The alterations of the NOSs is related to the development of hypertension, and hypertension has been known to induce the skeletal muscle changes including decreased fatigue resistance and contractile force. The purpose of this study was to observe the effect of IP on the expressions of the NOSs and Akt in the skeletal muscle of the spontaneously hypertensive rat (SHR). Nine weeks old male normotensive rat (Wistar-Kyoto rat, WKY) and SHR were divided into control and IP groups. The IP group was further divided into 3 or 10 cycles of IP. For IP, left common iliac artery was occluded 3 or 10 cycles of 5 min of ischemia/5 min of reperfusion using rodent vascular clamp. The animals were sacrificed at 0, 0.5, 1, 3 and 24 hours after reperfusion and the tibialis anterior muscles were removed. The expressions of nNOS, iNOS, eNOS, and Akt were examined with immunohistochemical methods and Western blot analysis. In the SHR, the expression of nNOS was decreased, iNOS was increased and the expressions of eNOS and AKT were decreased, compare to WKY. The expression of iNOS was increased, and nNOS, eNOS and Akt were decreased in SHR by 10IP. In summary, IP increased the expression of iNOS, and decreased the expression of eNOS and Akt in the SHR. It is suggested that hypertension may aggravate injury of the tibialis anterior muscle induced by repetitive ischemic conditioning.

KEYWORDS

Hypertension, Ischemic preconditioning, Tibialis anterior muscle, NOS, Akt

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[journal] Alderton WK / 2001 / Nitric oxide synthase: Structure, function and inhibition / Biochem J 357 : 593~615

[journal] Ameen M / 1991 / A comparison of free intracellular calcium and magnesium levels in the vascular smooth muscle and striated muscle cells of the spontaneously hypertensive and Wistar Kyoto normotensive rat / Ann NY Acad Sci 639 : 550~553

[journal] Brazil DP / 2001 / Ten years of protein kinase B signalling: a hard Akt to follow / Trends Biochem Sci 26 : 657~664

[journal] Chen II / 1981 / Microvascular rarefaction in spontaneously hypertensive rat cremaster muscle / Am J Physiol 241 : 306~310

[journal] Chou TC / 1998 / Alterations of nitric oxide synthase expression with aging and hypertension in rats / Hypertension 31 : 643~648

[journal] Forstermann U / 1986 / Stimulation of soluble guanylate cyclase by an acetylcholine-induced endothelium-derived factor from rabbit and canine arteries / Circ Res 58 : 531~538

[journal] Fujio Y / 2000 / Akt promotes survival of cardiomyocytes in vitro and protects against ischemia-reperfusion injury in mouse heart / Circulation 101 : 660~667

[journal] Gray SD / 1994 / Soleus muscle contractile properties in hypertensive rats / Am J Physiol Regul Integr Comp Physiol 267 : 735~739

[journal] Kelly KA / 1994 / Dimethyl sulfoxide modulates NF-kappa B and cytokine activation in lipopolysaccharide-treated murine macrophages / Infect Immun 62 : 3122~3128

[journal] Kis A / 2003 / Second window of protection following myocardial preconditioning: an essential role for PI3 kinase and p70S6 kinase / J Mol Cell Cardiol 35 : 1063~1071

[journal] 고봉철 / 2007 / Effects of Repetitive Ischemic Preconditioning on the Expression of Nitric Oxide Synthasein Tibialis Anterior and Soleus Muscles of the Rat / 대한체질인류학회지 / 대한체질인류학회 20(3) : 213~224

[journal] Krotkiewski M / 1998 / High blood pressure and muscle morphology/metabolismcausal relationship or only associated factors? / Clin Physiol 18 : 203~213

[journal] Lochner A / 2002 / Nitric oxide triggers classic ischemic preconditoning / Ann NY Acad Sci 962 : 402~414

[journal] Lockette W / 1986 / The loss of endotheliumdependent relaxation in hypertension / Hypertension 8 : 61~66

[journal] Muscari C / 2004 / Early preconditioning prevents the loss of endothelial nitric oxide synthase and enhances its activity in the ischemic/reperfused rat heart / Life Sci 74 : 1127~1137

[journal] Punkt K / 2002 / Myopathy-dependent changes in activity of ATPase, SDH and GPDH and NOS expression in the different fibre types of hamster muscles / Acta Histochem 104 : 15~22

[journal] Quadrilatero J / 2008 / Evidence for a pro-apoptotic phenotype in skeletal muscle of hypertensive rats / Biochem Biophys Res Commun 368 : 168~174

[journal] Quadrilatero J / 2006 / Increased DNA fragmentation and altered apoptotic protein levels in skeletal muscle of spontaneously hypertensive rats / J Appl Physiol 101 : 1149~1161

[journal] 서윤경 / 2006 / Expression Pattern of Nitric Oxide Synthases in Rat Skeletal Muscle after Cyclic Episodes of Short Ischemia and Reperfusion / 대한체질인류학회지 / 대한체질인류학회 19(2) : 125~136

[journal] Seo YK / 2003 / Age-related alterations of apoptosis in ischemic-reperfused rat tibialis anterior and soleus muscles. / Korean J Phys Anthropol 16 : 291~299

[journal] Smith LM / 2006 / Decreased supply-dependent oxygen consumption in the skeletal muscle of the spontaneously hypertensive rat during acute hypoxia / Shock 25 : 618~624

[journal] Tidball JG / 1998 / Mechanical loading regulates NOS expression and activity in developing and adult skeletal muscle / Am J Physiol 275 : 260~266

[journal] Tolins JP / 1990 / Role of endothelium-derived relaxing factor in regulation of renal hemodynamic responses / Am J Physiol 258 : 655~662

[journal] Tsui JC / 2008 / Nitric oxide synthase in critically ischaemic muscle and alterations in isoform expression during revascularization surgery / Br J Surg 95 : 72~79

[journal] Ushio-Fukai M / 1999 / Reactive oxygen species mediate the activation of Akt/protein kinase B by angiotensin II in vascular smooth muscle cells / J Biol Chem 274 : 22699~22704

[journal] Zhang L / 2003 / Reperfusion injury is reduced in skeletal muscle by inhibition of inducible nitric oxide synthase / J Appl Physiol 94 : 1473~1478

[journal] Zhou H / 2000 / Akt regulates cell survival and apoptosis at a postmitochondrial level / J Cell Biol 151 : 483~494

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Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Expressions of Nitric Oxide Synthases and Akt in the Tibialis Anterior Muscle of the Spontaneously Hypertensive Rat after Ischemic Preconditioning

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* References for papers published after 2023 are currently being built.

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Expressions of Nitric Oxide Synthases and Akt in the Tibialis Anterior Muscle of the Spontaneously Hypertensive Rat after Ischemic Preconditioning

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