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@article{ART001328889},
author={기미아 and Youn, Jeehee and 김정은 and 임우석 and 홍석만},
title={IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2009},
volume={22},
number={1},
pages={95-105},
doi={10.11637/aba.2009.22.1.95}

TY - JOUR
AU - 기미아
AU - Youn, Jeehee
AU - 김정은
AU - 임우석
AU - 홍석만
TI - IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System
JO - Anatomy & Biological Anthropology
PY - 2009
VL - 22
IS - 1
PB - 대한체질인류학회
SP - 95
EP - 105
SN - 2671-5651
AB - Interleukin-12 (IL-12), consisting of p35 and p40, plays important roles in linking innate and adaptive immunity. While p35 is constitutively expressed, IL-12 p40 gene expression is induced upon activation by Toll-like receptor ligands. Recently, with gene targeting technology, the cytokine IL-12 p40 reporter mouse has been developed to express the p40 gene linked via a viral IRES element with yellow fluorescence protein (YFP) fluorescent reporter. We investigated whether this novel system would be useful to reveal IL-12 p40-producing immune cells. We first investigated whether macrophages and dendritic cells from these mice faithfully reported p40 induction. Next, we tested if microglial cells, macrophages in the brain, could induce IL-12 p40. Finally we tested whether B cells could produce IL-12 p40 because there were very few reports for IL-12 production by B cells. Our results confirmed that macrophages and dendritic cells are main producer of IL-12 p40. Then, we found that microglial cells could produce IL-12 p40 upon stimulation with various TLR ligands. Finally we found that a subset of B cells could produce IL-12 p40 in TLR9-dependent manner. Taken all together, our system will be a valuable tool to identify the type of immune cells that produce IL-12 p40.
KW - Fluorescent reporter mouse system;Interleukin-12;Toll-like receptor;Microglia
DO - 10.11637/aba.2009.22.1.95
ER -

기미아, Youn, Jeehee, 김정은, 임우석 and 홍석만. (2009). IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System. Anatomy & Biological Anthropology, 22(1), 95-105.

기미아, Youn, Jeehee, 김정은, 임우석 and 홍석만. 2009, "IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System", Anatomy & Biological Anthropology, vol.22, no.1 pp.95-105. Available from: doi:10.11637/aba.2009.22.1.95

기미아, Youn, Jeehee, 김정은, 임우석, 홍석만 "IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System" Anatomy & Biological Anthropology 22.1 pp.95-105 (2009) : 95.

기미아, Youn, Jeehee, 김정은, 임우석, 홍석만. IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System. 2009; 22(1), 95-105. Available from: doi:10.11637/aba.2009.22.1.95

기미아, Youn, Jeehee, 김정은, 임우석 and 홍석만. "IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System" Anatomy & Biological Anthropology 22, no.1 (2009) : 95-105.doi: 10.11637/aba.2009.22.1.95

기미아; Youn, Jeehee; 김정은; 임우석; 홍석만. IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System. Anatomy & Biological Anthropology, 22(1), 95-105. doi: 10.11637/aba.2009.22.1.95

기미아; Youn, Jeehee; 김정은; 임우석; 홍석만. IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System. Anatomy & Biological Anthropology. 2009; 22(1) 95-105. doi: 10.11637/aba.2009.22.1.95

기미아, Youn, Jeehee, 김정은, 임우석, 홍석만. IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System. 2009; 22(1), 95-105. Available from: doi:10.11637/aba.2009.22.1.95

기미아, Youn, Jeehee, 김정은, 임우석 and 홍석만. "IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System" Anatomy & Biological Anthropology 22, no.1 (2009) : 95-105.doi: 10.11637/aba.2009.22.1.95