Expression of DNA Repairing Enzymes in the Cerebral Tissue of the Rat Fetus After Hypoxic Injury (흰쥐 태아 대뇌조직에서 저산소증 손상에 의한 DNA 복구 효소의 발현 변화)

이경진; Jung-Woo Kim (김정우); 민병운; 배난영; 강기영

doi:10.11637/aba.2012.25.2.67

Expression of DNA Repairing Enzymes in the Cerebral Tissue of the Rat Fetus After Hypoxic Injury

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2012, 25(2), pp.67~76
DOI : 10.11637/aba.2012.25.2.67
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

이경진 ¹, Jung-Woo Kim ¹, 민병운 ², 배난영 ³, 강기영 ¹

¹서남대학교
²한려대학교
³광양보건대학교

Accredited

ABSTRACT

Hypoxia is one of the major causes of neonatal mortality. Hypoxia-induced tissue injuries are resulted from complex mechanisms such as DNA damage and apoptosis. In this study, we aimed to elucidate the changes in the expression of DNA repairing enzymes such as 8-hydroxyguanine glycosylase 1 (OGG1) and apurinic/apyrimidinic endonuclease 1 (APE1) and brain derived neurotrophic factor (BDNF) in the fetal cerebral tissue after intrauterine hypoxic injury. For this study, pregnant Sprague-Dawley rats were exposed to hypoxic gas (10% O2, 5% CO2, 85% N2) for 2 or 4 hours at postconception day 14.5 and 15.5. After 24 hours, the animals were anesthetized with ethyl ether and fetuses were obtained by laparatomy. Hematoxylin-eosin stain, immunohistochemical stain, and western blot were employed for analysis. The caspase-3 immunolabeled cells were significantly increased within the cerebral cortex after hypoxic injury. The expressions of OGG1, APE1, and BDNF were also increased in the cerebral tissue after hypoxic injury at postconception day 14.5, in a dose-dependent manner. However, the expression of BDNF was significantly decreased in the cortical tissue exposed to hypoxic injury at postconception day 15.5. These results demonstrate that fetal hypoxic injury induces apoptosis of the nerve cells and promotes the expressions of the DNA repairing enzymes and neurotrophic factors. In addition, these results suggest that protection mechanisms against hypoxic injury alter along the progression of the fetal development.

KEYWORDS

Hypoxia, Apoptosis, OGG1, APE1, BDNF

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Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Expression of DNA Repairing Enzymes in the Cerebral Tissue of the Rat Fetus After Hypoxic Injury

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Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Expression of DNA Repairing Enzymes in the Cerebral Tissue of the Rat Fetus After Hypoxic Injury

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