Carcinogenic Compounds, 2-nitrofluorene and Clonidine Can Modulate the Level of Sirtuin mRNAs Expression in Human Adipose Tissue-derived Stem Cells (발암물질인 2-nitrofluorene와 clonidine은 사람지방줄기세포의 sirtuin mRNAs의 발현의 정도를 조절한다)

Jiyoun Kim (김지연); 이채영; Young-Ho Kwon (권영호); Ho Chan Kim (김호찬); 김현

doi:10.11637/aba.2014.27.4.235

Carcinogenic Compounds, 2-nitrofluorene and Clonidine Can Modulate the Level of Sirtuin mRNAs Expression in Human Adipose Tissue-derived Stem Cells

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2014, 27(4), pp.235~243
DOI : 10.11637/aba.2014.27.4.235
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

Jiyoun Kim ¹, 이채영 ¹, Young-Ho Kwon ¹, Ho Chan Kim ¹, 김현 ¹

¹고신대학교

Accredited

ABSTRACT

Sirtuins (SIRTs) are involved in multiple cellular processes. And they are involved in cellular pathwaysrelated aging, cancer, and a variety of cellular functions including cell cycle, DNA repair and proliferation. Also they modulate life span. Stem cells have the ability to self-renew for unlimited proliferation and differentiateinto various cell types. It has been a little known that the mutation of undifferentiated stem cells in tissue may resultin the development of cancer cells by genotoxic carcinogens. Therefore, this study investigated whether somecarcinogenic compounds can modulate the expression of sirtuin mRNA on human adipose-derived stem cells. Adipose-derived stem cells (ADSC) were exposed to genotoxic carcinogenic compound (2-nitrofluorene, 2NF)and non-genotoxic carcinogenic compound (clonidine, CND) for 24 hours, 48 hours, 72 hours and 96 hours. Theexpression of SIRT1 mRNA increased on 72 hours. Expressions of SIRT2 and SIRT7 mRNA increased robustlyon 48 hours. But all of SIRTs decreased to a level before a treatment of genotoxic compound on adipose-derivedstem cells. These results demonstrated that a treatment of genotoxic compound induced the expression of SIRT mRNA onlyin the short time. But their level returned to untreated cells on 96 hours. They suggest that the possibility that thesirtuins can retard the carcinogenesis of adipose derived stem cells.

KEYWORDS

Sirtuin, Human adipose derived stem cells, 2-nitrofluorene, Clonidine

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Anatomy & Biological Anthropology 2024 KCI Impact Factor : 0.36

Carcinogenic Compounds, 2-nitrofluorene and Clonidine Can Modulate the Level of Sirtuin mRNAs Expression in Human Adipose Tissue-derived Stem Cells

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Anatomy & Biological Anthropology 2024 KCI Impact Factor : 0.36

Carcinogenic Compounds, 2-nitrofluorene and Clonidine Can Modulate the Level of Sirtuin mRNAs Expression in Human Adipose Tissue-derived Stem Cells

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