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@article{ART002264961},
author={BaeChoonsang and 조혜정 and AHN KYU YOUN},
title={Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2017},
volume={30},
number={3},
pages={87-98},
doi={10.11637/aba.2017.30.3.87}

TY - JOUR
AU - BaeChoonsang
AU - 조혜정
AU - AHN KYU YOUN
TI - Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat
JO - Anatomy & Biological Anthropology
PY - 2017
VL - 30
IS - 3
PB - 대한체질인류학회
SP - 87
EP - 98
SN - 2671-5651
AB - Hypokalemia causes metabolic alkalosis and morphological changes of the kidney. K+ balance is regulated not only by ion channels or pump gene, but also by various genes including NF-E2-related factor 2 (Nrf2). Previous study suggested the possibility that Akt and ERK kinase may be involved in Nrf2 transcriptional gene activation. In present study, we investigate the alterations of Akt, p-Akt, ERK, p-ERK protein in both normal kidney and K+-deficient diet kidney using Western blot analysis, and immunohistochemisrty. Our western blot data showed that the expression of Akt and p-Akt was increased gradually in K+-depleted diet (from 1W-3W) compared to normal group. The expression of ERK and p-ERK was markedly increased in K+-depleted diet 2W in comparison with normal group. Based on our immunostaining results, Akt protein immunoreactivity was prominently increased in outer medullary collecting duct, especially in K+-depleted diet 2 weeks. The localization of p-Akt proteins in K+-depleted groups was not different from normal group, but the immunoreactivity was significantly increased in distal convoluted tubule, macula densa and outer medullary thick ascending limb in K+-depleted diet 1 and 2 weeks groups. ERK protein immunoreactivity was prominently increased in outer medullary collecting duct, especially in K+-depleted diet 2 and 3 weeks. The localization of p-ERK proteins in K+-depleted groups was not different from normal group, but the immunoreactivity was prominently increased in the nucleus of outer medullary collecting duct especially in K+-depleted diet 2 weeks. Taken together, we suggest that the expression of p-Akt was gradually increased in K+-depleted groups of kidney, but the expression of p-ERK was markedly increased in K+-depleted diet 2 week group. Hence, the promotion of AKT and ERK phosphorylation in hypokalemic condition may be involved in the regulation of ion channels, ion transporters and subsequent intracellular signal transduction.
KW - Hypokalemia;Metabolic alkalosis;Akt;p-Akt;ERK;p-ERK;Immunohistochemisty
DO - 10.11637/aba.2017.30.3.87
ER -

BaeChoonsang, 조혜정 and AHN KYU YOUN. (2017). Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat. Anatomy & Biological Anthropology, 30(3), 87-98.

BaeChoonsang, 조혜정 and AHN KYU YOUN. 2017, "Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat", Anatomy & Biological Anthropology, vol.30, no.3 pp.87-98. Available from: doi:10.11637/aba.2017.30.3.87

BaeChoonsang, 조혜정, AHN KYU YOUN "Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat" Anatomy & Biological Anthropology 30.3 pp.87-98 (2017) : 87.

BaeChoonsang, 조혜정, AHN KYU YOUN. Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat. 2017; 30(3), 87-98. Available from: doi:10.11637/aba.2017.30.3.87

BaeChoonsang, 조혜정 and AHN KYU YOUN. "Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat" Anatomy & Biological Anthropology 30, no.3 (2017) : 87-98.doi: 10.11637/aba.2017.30.3.87

BaeChoonsang; 조혜정; AHN KYU YOUN. Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat. Anatomy & Biological Anthropology, 30(3), 87-98. doi: 10.11637/aba.2017.30.3.87

BaeChoonsang; 조혜정; AHN KYU YOUN. Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat. Anatomy & Biological Anthropology. 2017; 30(3) 87-98. doi: 10.11637/aba.2017.30.3.87

BaeChoonsang, 조혜정, AHN KYU YOUN. Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat. 2017; 30(3), 87-98. Available from: doi:10.11637/aba.2017.30.3.87

BaeChoonsang, 조혜정 and AHN KYU YOUN. "Alteration of Akt, p-Akt, ERK, and p-ERK Proteins Expression in the Kidney of Hypokalemic Rat" Anatomy & Biological Anthropology 30, no.3 (2017) : 87-98.doi: 10.11637/aba.2017.30.3.87