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Kinetic evaluation and study of gold-based nanoparticles and multi-walled carbon nanotubes as an alkaline phosphatase inhibitor in serum and pure form

  • Carbon Letters
  • Abbr : Carbon Lett.
  • 2023, 33(4), pp.1217-1229
  • DOI : 10.1007/s42823-023-00509-z
  • Publisher : Korean Carbon Society
  • Research Area : Natural Science > Natural Science General > Other Natural Sciences General
  • Received : December 14, 2022
  • Accepted : March 25, 2023
  • Published : June 1, 2023

Mohammed Samar Naser 1 Al-Rawi Khalid F. 1 Mohammed Ahmed Mishaal 1

1University of Anbar, Ramadi

Accredited

ABSTRACT

In this study, gold nanoparticles (AuNPs) were synthesised using green chemistry to decorate multi-walled carbon nanotubes (MWCNTs) made from walnut shells transmission electron microscopy, field-emission scanning electron microscopy (FESEM), atomic force microscopy and fourier transforms infrared spectroscopy were used to diagnose MWCNTs and AuNPs. MWCNT-COOAu, MWCNT-COO and MWCNT-Au were diagnosed by Raman, energy dispersive X-ray analysis and FESEM. The effect of AuNPs, MWCNT-COO, MWCNT-COOAu and MWCNT-Au on pure and serum alkaline phosphatase (ALP) enzyme activity was studied in vitro using the enzyme-substrate 4-nitrophenyl disodium orthophosphate. For pure enzymes, Vmax slightly increased as the concentration of MWCNT-Au, MWCNT-COOAu and MWCNTCOO increased, whereas the Vmax values decreased as the concentration of AuNPs increased. The inhibition type for all NPs varied. For serum ALP enzyme, the Vmax values for Au-based NPs decreased as the concentration of NPs increased. The Vmax values exceeded the standard value at the concentrations of 25, 50 and 75 ppm for MWCNT-Au and MWCNT-COOAu, whereas the Vmax values increased over the standard value for all concentrations of AuNPs.

Citation status

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Total Citation Counts(KCI+WOS) (8) This is the number of times that the duplicate count has been removed by comparing the citation list of WoS and KCI.

Scopus Citation Counts (8) This is the result of checking the information with the same ISSN, publication year, volume, and start page between articles in KCI and the SCOPUS journals. (as of 2024-10-01)

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