Quantum dot nanocomposite-based luminescent materials have gained attention for solid-state lighting and optical displays. This study presents a one-step, eco-friendly hydrothermal process to synthesize nitrogen, potassium, and calcium-doped carbon quantum dots (N, K, Ca-doped CQDs) from the flower extract of Mesembryanthemum crystallinum L. (ice plant). The CQDs were characterized using HRTEM, EDX, SAED, XPS, XRD, NMR, FTIR, zeta potential, UV–Vis, and photoluminescence spectroscopy. HRTEM revealed an average particle size of 4.6 nm, with a range of 2 to 7 nm. The CQDs exhibited a quantum yield of 20%, excellent water solubility, photostability, and greenish fluorescence under UV (365 nm). The fluorescence spectra were analyzed using CIE (Commission Internationale de l’Eclairage) chromaticity coordinates to determine the emitted color. The fluorescence emission behavior was influenced by solvent polarity, locally excited (LE) states, intramolecular charge transfer (ICT) processes, and hydrogen bonding. The hydrogen bonds between N, K, Ca-doped CQDs and DI water likely enhanced the stability of the ICT state, resulting in a red shift in fluorescence. Additionally, we developed an eco-friendly wheat-starch-based bioplastic nanocomposite by embedding the CQDs. The effects of CQD concentration and pH sensitivity on luminescent properties were explored. Finally, we demonstrated a practical application by designing a conceptual nameplate-like calligraphy using the optimized CQDs@bioplastic nanocomposite film (CQD concentration: 240 mg/mL, pH: 2.7), highlighting its potential for luminescent film applications.