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An electrochemical sensor based on graphene-chitosan-cyclodextrin modification for the detection of Staphylococcus aureus

  • Carbon Letters
  • Abbr : Carbon Lett.
  • 2024, 34(1), pp.495~504
  • DOI : 10.1007/s42823-023-00518-y
  • Publisher : Korean Carbon Society
  • Research Area : Natural Science > Natural Science General > Other Natural Sciences General
  • Received : February 21, 2023
  • Accepted : April 13, 2023
  • Published : February 1, 2024

Li Guochun 1 Li Jiaxin 2 Yang Ziyu 3

1Department of Infectious Diseases, Wangcang People’s Hospital
2Department of Cardiovascular Medicine, Wangcang Hospital of Traditional Chinese Medicine
3Department of Respiratory and Critical Care Medicine, Wangcang County People’s Hospital

Accredited

ABSTRACT

This study reports the synthesis of a novel graphene/chitosan/β-cyclodextrin composite material (GO/CS/β-CD) via a one-step chemical reduction method, which combines the advantages of graphene, chitosan, and β-cyclodextrin. The morphology and structure of the composite were characterized using various techniques, such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. Subsequently, sortase A (SA) was immobilized onto the GO/CS/β-CD for the detection of Staphylococcus aureus. The sensor exhibited a good linear relationship within the concentration range of 30–300 CFU/mL, with a detection limit of 12 CFU/mL. The GO/CS/β-CD composite material showed enhanced properties due to the synergistic effect of graphene, chitosan, and β-cyclodextrin. The immobilization of sortase A onto the composite material improved the sensitivity and selectivity of the sensor for the detection of S. aureus. This study presents a novel graphene/chitosan/β-cyclodextrin composite material with immobilized sortase A, demonstrating enhanced sensitivity and selectivity for the detection of Staphylococcus aureus, which has potential for the development of high-performance sensors in various fields.

Citation status

Scopus Citation Counts (6) This is the result of checking the information with the same ISSN, publication year, volume, and start page between articles in KCI and the SCOPUS journals. (as of 2025-01-01)

* References for papers published after 2023 are currently being built.