Anticancer(AC)-Functional Kimchi Exhibits Antiobesity Effects in Differentiated 3T3-L1 Adipocytes

Eui-Seong Park; Seung-Min Lee; Yaung-lee Lim; Kun-Young Park

Anticancer(AC)-Functional Kimchi Exhibits Antiobesity Effects in Differentiated 3T3-L1 Adipocytes

CELLMED
Abbr : CellMed
2018, 8(3), pp.11-11
Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
Research Area : Medicine and Pharmacy > General Medicine

Eui-Seong Park ¹, Seung-Min Lee ¹, Yaung-lee Lim ², Kun-Young Park ³

¹Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seodaemun-gu, Seoul, 03722, Republic of Korea
²Department of Food and Nutrition, Sungshin women’s University, Seungbuk-gu, Seoul, 01133, Republic of Korea
³Department of Food Science and Biotechnology, Cha University, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea

Candidate

ABSTRACT

In vitro anti-obesity effects of anti-cancer (AC) functional kimchi in differentiated 3T3-L1 adipocytes were studied. We constructed three experimental groups: Control, standardized kimchi (SK), and AC functional kimchi (A-FK) that included active ingredients and Lactobacillus plantarum. Kimchi extracts did not show any cytotoxicity in pre-adipocytes in the concentration range of 1 – 5 mg/mL. A-FK significantly reduced fat droplet formation and absorbance in differentiated 3T3-L1 adipocytes, as shown by Oil red O staining, compared to Control and SK (P < 0.05). SK and A-FK reduced adipo-/lipogenesis related genes such as C/EBPα, SREBP-1, LPL, and LXRα compared to Control (P < 0.05). Especially, A-FK more greatly reduced SREBP-1 and LPL compared to SK (P < 0.05). A-FK up-regulated the β-oxidation related gene CPT-1c and down-regulated the pro-inflammatory cytokine IL-6 compared to Control (P < 0.05). Based on the results, A-FK exhibited anti-obesity effects by inhibiting fat droplet formation and adipo-/lipogenesis related genes by regulating the β-oxidation related gene CPT-1c and pro-inflammatory cytokine IL-6. In previous studies, A-FK kimchi already exhibited a strong anti-cancer effect. These results indicate that A-FK increased anti-obesity activity in this model system due to its functional ingredients and anti-cancer functionality.

KEYWORDS

Anti-obestiy, kimchi, Adipogenesis, Lipogenesis 3T3-L1 adipocyte

Citation status

* References for papers published after 2023 are currently being built.

[journal] Cowherd RM / 1999 / Molecular regulation of adipocyte differentiation / Seminars in cell & developmental biology 10 : 3~10

[journal] Meizi Cui / 2015 / Antiobesity effects of kimchi in diet-induced obese mice / Journal of Ethnic Foods / 한국식품연구원 2(3) : 137~144

[journal] Duplus E / 2000 / Fatty acid regulation of gene transcription / J Biol Chem 275 : 30749~30752

[journal] Gerlier D / 1986 / Use of MTT colorimetric assay to measure cell activation / J Immunol Methods 94 : 57~63

[journal] Irie J / 1997 / Inhibition of lipid accumulation in lipomyces yeast by 2 (E), 4 (E)-decadienoic acid from pepper / Food Sci Technol Int 3 : 409~413

[journal] Janeway Jr CA. Medzhitov R / 2002 / Innate immune recognition / Annual Rev Immunol 20 : 197~216

[journal] Jeong M / 2015 / Dietary prevention of Helicobacter pyloriassociated gastric cancer with kimchi / Oncotarget 6 : 29513~29526

[journal] 주민정 / 2009 / Physiological Activities of Mistletoe Extracts from Viscum album L. / 한국식품영양과학회지 / 한국식품영양과학회 38(5) : 529~534

[journal] Jung HY / 2013 / The Korean mistletoe (Viscum album coloratum) extract has an antiobesity effect and protects against hepatic steatosis in mice with high-fat diet-induced obesity / Evidence-Based Complementary and Alternative Med : 1~9

[journal] 김보경 / 2015 / Anticancer Effects of Kimchi Fermented for Different Times and with Added Ingredients in Human HT-29 Colon Cancer Cells / Food Science and Biotechnology / 한국식품과학회 24(2) : 629~633

[journal] Kim EK / 2011 / Fermented kimchi reduces body weight and improves metabolic parameters in overweight and obese patients / Nutr Res 31 : 436~443

[thesis] Kim HY / 2013 / Fermentation properties and inhibitory effects of anticancer functional baechu kimchi on AOM/DSS induced colitis-associated colon cancer in Balb/c mice / Pusan National University

[journal] Kim HY / 2018 / Clinical trials of kimchi intakes on the regulation of metabolic parameters and colon health in healthy Korean young adults / J Function Food 47 : 325~333

[journal] 김희영 / 2014 / Kimchi Protects Against Azoxymethane/Dextran Sulfate Sodium–Induced Colorectal Carcinogenesis in Mice / Journal of Medicinal Food / 한국식품영양과학회 17(8) : 833~841

[journal] 이경희 / 2015 / Anti-Obesity Effects of Starter Fermented Kimchi on 3T3-L1 Adipocytes / Preventive Nutrition and Food Science / 한국식품영양과학회 20(4) : 298~302

[journal] 이현아 / 2015 / Effect of Nanometric Lactobacillus plantarum in Kimchi on Dextran Sulfate Sodium-Induced Colitis in Mice / Journal of Medicinal Food / 한국식품영양과학회 18(10) : 1073~1080

[journal] 이현아 / 2015 / Dead Nano-Sized Lactobacillus plantarum Inhibits Azoxymethane/Dextran Sulfate Sodium-Induced Colon Cancer in Balb/c Mice / Journal of Medicinal Food / 한국식품영양과학회 18(12) : 1400~1405

[journal] Livak KJ / 2001 / Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta C(T)) method / Methods 25 : 402~408

[journal] Magana MM / 1996 / Two tandem binding sites for sterol regulatory element binding proteins are required for sterol regulation of fatty-acid synthase promoter / J Biol Chem 271 : 32689~32694

[journal] Mead JR / 2002 / Lipoprotein lipase: structure, function, regulation, and role in disease / J Mol Med 80 : 753~769

[journal] Moro, CO / 2000 / Obesity and medicinal plants / Fitoterapia 71 : 73~82

[journal] Nguyen PH / 2016 / Anti-inflammatory terpenylated coumarins from the leaves of Zanthoxylum schinifolium with <TEX>${\alpha}$</TEX>-glucosidase inhibitory activity / J Natural Med 70 : 276~281

[journal] 박의성 / 2018 / In Vitro Anti-Obesity Effects of Kimchi Prepared with Solar Salts without Bittern on 3T3-L1 Adipocytes / 한국식품영양과학회지 / 한국식품영양과학회 47(2) : 229~234

[journal] Park JA / 2012 / Anti-obesity effect of kimchi fermented with Weissella koreensis OK1-6 as starter in high-fat diet-induced obese C57BL/6J mice / J Applied Microbiol 113 : 1507~1516

[journal] 박건영 / 2014 / Health Benefits of Kimchi (Korean Fermented Vegetables) as a Probiotic Food / Journal of Medicinal Food / 한국식품영양과학회 17(1) : 6~20

[journal] Rocha VZ / 2009 / Obesity, inflammation, and atherosclerosis / Nature Rev Cardiol 6 : 399~409

[journal] Schmuth M / 2008 / Thematic review series: skin lipids. Peroxisome proliferatoractivated receptors and liver X receptors in epidermal biology / J Lipid Res 49 : 499~509

[thesis] Song GH / 2018 / Anticancer Effects of kimchi prepared with Chungang baechu cabbage in human cancer cells and AOM/DSS induced colitis-associated colorectal cancer mice / Yonsei University

[journal] Takemura N / 2010 / Lactobacillus plantarum strain No. 14 reduces adipocyte size in mice fed high-fat diet / Exp Biol Med 235 : 849~856

[journal] Wu Z / 1999 / Transcriptional activation of adipogenesis / Current Opinion Cell Biol 11 : 689~694

[journal] Xu N / 2014 / Low-dose diet supplement of a natural flavonoid, luteolin, ameliorates diet-induced obesity and insulin resistance in mice / Mol Nutr Food Res 58 : 1258~1268

[journal] Yokoyama C / 1993 / SREBP-1, a basic-helix-loop-helixleucine zipper protein that controls transcription of the low density lipoprotein receptor gene / Cell 75 : 187~197

[thesis] Yoon JY / 2005 / Studies on enhancement of antiobesitic effects of beachu kimchi and baek-kimchi / Pusan National University

[journal] Yun JW / 2010 / Possible anti-obesity therapeutics from nature-A review / Phytochem 71 : 1625~1641

[journal] Zebisch K / 2012 / Protocol for effective differentiation of 3T3-L1 cells to adipocytes / Analy Biochem 425 : 88~90

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