Anticancer(AC)-Functional Kimchi Exhibits Antiobesity Effects in Differentiated 3T3-L1 Adipocytes

Eui-Seong Park; Seung-Min Lee; Yaung-lee Lim; Kun-Young Park

Anticancer(AC)-Functional Kimchi Exhibits Antiobesity Effects in Differentiated 3T3-L1 Adipocytes

CELLMED
Abbr : CellMed
2018, 8(3), pp.11~11
Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
Research Area : Medicine and Pharmacy > General Medicine

Eui-Seong Park ¹, Seung-Min Lee ¹, Yaung-lee Lim ², Kun-Young Park ³

¹Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seodaemun-gu, Seoul, 03722, Republic of Korea
²Department of Food and Nutrition, Sungshin women’s University, Seungbuk-gu, Seoul, 01133, Republic of Korea
³Department of Food Science and Biotechnology, Cha University, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea

Candidate

ABSTRACT

In vitro anti-obesity effects of anti-cancer (AC) functional kimchi in differentiated 3T3-L1 adipocytes were studied. We constructed three experimental groups: Control, standardized kimchi (SK), and AC functional kimchi (A-FK) that included active ingredients and Lactobacillus plantarum. Kimchi extracts did not show any cytotoxicity in pre-adipocytes in the concentration range of 1 – 5 mg/mL. A-FK significantly reduced fat droplet formation and absorbance in differentiated 3T3-L1 adipocytes, as shown by Oil red O staining, compared to Control and SK (P < 0.05). SK and A-FK reduced adipo-/lipogenesis related genes such as C/EBPα, SREBP-1, LPL, and LXRα compared to Control (P < 0.05). Especially, A-FK more greatly reduced SREBP-1 and LPL compared to SK (P < 0.05). A-FK up-regulated the β-oxidation related gene CPT-1c and down-regulated the pro-inflammatory cytokine IL-6 compared to Control (P < 0.05). Based on the results, A-FK exhibited anti-obesity effects by inhibiting fat droplet formation and adipo-/lipogenesis related genes by regulating the β-oxidation related gene CPT-1c and pro-inflammatory cytokine IL-6. In previous studies, A-FK kimchi already exhibited a strong anti-cancer effect. These results indicate that A-FK increased anti-obesity activity in this model system due to its functional ingredients and anti-cancer functionality.

KEYWORDS

Anti-obestiy, kimchi, Adipogenesis, Lipogenesis 3T3-L1 adipocyte

Journal Copyright Policy

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.

Citation status

* References for papers published after 2025 are currently being built.

[journal] Cowherd RM / 1999 / Molecular regulation of adipocyte differentiation / Seminars in cell & developmental biology 10 : 3~10

[journal] Meizi Cui / 2015 / Antiobesity effects of kimchi in diet-induced obese mice / Journal of Ethnic Foods / 한국식품연구원 2(3) : 137~144

[journal] Duplus E / 2000 / Fatty acid regulation of gene transcription / J Biol Chem 275 : 30749~30752

[journal] Gerlier D / 1986 / Use of MTT colorimetric assay to measure cell activation / J Immunol Methods 94 : 57~63

[journal] Irie J / 1997 / Inhibition of lipid accumulation in lipomyces yeast by 2 (E), 4 (E)-decadienoic acid from pepper / Food Sci Technol Int 3 : 409~413

[journal] Janeway Jr CA. Medzhitov R / 2002 / Innate immune recognition / Annual Rev Immunol 20 : 197~216

[journal] Jeong M / 2015 / Dietary prevention of Helicobacter pyloriassociated gastric cancer with kimchi / Oncotarget 6 : 29513~29526

[journal] 주민정 / 2009 / Physiological Activities of Mistletoe Extracts from Viscum album L. / 한국식품영양과학회지 / 한국식품영양과학회 38(5) : 529~534

[journal] Jung HY / 2013 / The Korean mistletoe (Viscum album coloratum) extract has an antiobesity effect and protects against hepatic steatosis in mice with high-fat diet-induced obesity / Evidence-Based Complementary and Alternative Med : 1~9

[journal] 김보경 / 2015 / Anticancer Effects of Kimchi Fermented for Different Times and with Added Ingredients in Human HT-29 Colon Cancer Cells / Food Science and Biotechnology / 한국식품과학회 24(2) : 629~633

[journal] Kim EK / 2011 / Fermented kimchi reduces body weight and improves metabolic parameters in overweight and obese patients / Nutr Res 31 : 436~443

[thesis] Kim HY / 2013 / Fermentation properties and inhibitory effects of anticancer functional baechu kimchi on AOM/DSS induced colitis-associated colon cancer in Balb/c mice / Pusan National University

[journal] Kim HY / 2018 / Clinical trials of kimchi intakes on the regulation of metabolic parameters and colon health in healthy Korean young adults / J Function Food 47 : 325~333

[journal] 김희영 / 2014 / Kimchi Protects Against Azoxymethane/Dextran Sulfate Sodium–Induced Colorectal Carcinogenesis in Mice / Journal of Medicinal Food / 한국식품영양과학회 17(8) : 833~841

[journal] 이경희 / 2015 / Anti-Obesity Effects of Starter Fermented Kimchi on 3T3-L1 Adipocytes / Preventive Nutrition and Food Science / 한국식품영양과학회 20(4) : 298~302

[journal] 이현아 / 2015 / Effect of Nanometric Lactobacillus plantarum in Kimchi on Dextran Sulfate Sodium-Induced Colitis in Mice / Journal of Medicinal Food / 한국식품영양과학회 18(10) : 1073~1080

[journal] 이현아 / 2015 / Dead Nano-Sized Lactobacillus plantarum Inhibits Azoxymethane/Dextran Sulfate Sodium-Induced Colon Cancer in Balb/c Mice / Journal of Medicinal Food / 한국식품영양과학회 18(12) : 1400~1405

[journal] Livak KJ / 2001 / Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta C(T)) method / Methods 25 : 402~408

[journal] Magana MM / 1996 / Two tandem binding sites for sterol regulatory element binding proteins are required for sterol regulation of fatty-acid synthase promoter / J Biol Chem 271 : 32689~32694

[journal] Mead JR / 2002 / Lipoprotein lipase: structure, function, regulation, and role in disease / J Mol Med 80 : 753~769

[journal] Moro, CO / 2000 / Obesity and medicinal plants / Fitoterapia 71 : 73~82

[journal] Nguyen PH / 2016 / Anti-inflammatory terpenylated coumarins from the leaves of Zanthoxylum schinifolium with <TEX>${\alpha}$</TEX>-glucosidase inhibitory activity / J Natural Med 70 : 276~281

[journal] 박의성 / 2018 / In Vitro Anti-Obesity Effects of Kimchi Prepared with Solar Salts without Bittern on 3T3-L1 Adipocytes / 한국식품영양과학회지 / 한국식품영양과학회 47(2) : 229~234

[journal] Park JA / 2012 / Anti-obesity effect of kimchi fermented with Weissella koreensis OK1-6 as starter in high-fat diet-induced obese C57BL/6J mice / J Applied Microbiol 113 : 1507~1516

[journal] 박건영 / 2014 / Health Benefits of Kimchi (Korean Fermented Vegetables) as a Probiotic Food / Journal of Medicinal Food / 한국식품영양과학회 17(1) : 6~20

[journal] Rocha VZ / 2009 / Obesity, inflammation, and atherosclerosis / Nature Rev Cardiol 6 : 399~409

[journal] Schmuth M / 2008 / Thematic review series: skin lipids. Peroxisome proliferatoractivated receptors and liver X receptors in epidermal biology / J Lipid Res 49 : 499~509

[thesis] Song GH / 2018 / Anticancer Effects of kimchi prepared with Chungang baechu cabbage in human cancer cells and AOM/DSS induced colitis-associated colorectal cancer mice / Yonsei University

[journal] Takemura N / 2010 / Lactobacillus plantarum strain No. 14 reduces adipocyte size in mice fed high-fat diet / Exp Biol Med 235 : 849~856

[journal] Wu Z / 1999 / Transcriptional activation of adipogenesis / Current Opinion Cell Biol 11 : 689~694

[journal] Xu N / 2014 / Low-dose diet supplement of a natural flavonoid, luteolin, ameliorates diet-induced obesity and insulin resistance in mice / Mol Nutr Food Res 58 : 1258~1268

[journal] Yokoyama C / 1993 / SREBP-1, a basic-helix-loop-helixleucine zipper protein that controls transcription of the low density lipoprotein receptor gene / Cell 75 : 187~197

[thesis] Yoon JY / 2005 / Studies on enhancement of antiobesitic effects of beachu kimchi and baek-kimchi / Pusan National University

[journal] Yun JW / 2010 / Possible anti-obesity therapeutics from nature-A review / Phytochem 71 : 1625~1641

[journal] Zebisch K / 2012 / Protocol for effective differentiation of 3T3-L1 cells to adipocytes / Analy Biochem 425 : 88~90

KJCKorea
Journal Central

CELLMED 2025 KCI Impact Factor : 0.18