Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS

Bong-Keun Jang (장봉근); Sunyoung Park (박선영); Dahyun Mun (문다현); Gunwoo Lee (이건우); Youngsun Kwon (권영선); Hye-yeon Kang (강혜연); Jeom-Yong Kim (김점용)

doi:10.5667/CellMed.2023.007

Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS

CELLMED
Abbr : CellMed
2023, 13(6), pp.7-7
DOI : 10.5667/CellMed.2023.007
Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
Research Area : Medicine and Pharmacy > General Medicine
Received : May 11, 2023
Accepted : May 19, 2023
Published : May 31, 2023

Bong-Keun Jang ¹, Sunyoung Park ¹, Dahyun Mun ¹, Gunwoo Lee ¹, Youngsun Kwon ¹, Hye-yeon Kang ¹, Jeom-Yong Kim ¹

¹JBK-LAB, Inc.

Accredited

ABSTRACT

Raphanus sativus L. has been reported to have anti-inflammatory and anti-tumor activity. However, the anti-inflammatory effect and mechanism of action of the Raphanus sativus L. seeds (RSS) with or without oil are still unknown. This study was undertaken to investigate the in-vitro anti-inflammatory effect with or without oil in the RSS on RAW 264.7 cells stimulated by lipopolysaccharide (LPS). Results showed the suppressed LPS-induced secretion of pro-inflammatory mediators such as nitric oxide (NO), inflammatory cytokine (IL-6, TNF-α). Additionally, a decrease in protein expression of iNOS was observed, but nuclear translocation of NF-κB p65 was not inhibited. To elucidate the underlying mechanism of the anti-inflammatory effect of RSS, the involvement of mitogen-activated protein kinase (MAPK) signaling pathways was examined. We also found that RSS blocked LPS-induced phosphorylation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK) signaling but did not affect the phosphorylation of p38 MAPK and extracellular signal-regulated kinase (ERK) 1/2. These results suggest that RSS may have potential as an anti-inflammatory agent through the inhibition of LPS-induced inflammatory cytokine production via regulation of the JNK pathway.

KEYWORDS

Raphanus sativus L. seed, anti-inflammation, macrophage, NO

Citation status

* References for papers published after 2023 are currently being built.

[journal] Jinah Hwang / 2016 / Bioconverted Jeju Hallabong tangor (Citrus kiyomi × ponkan) peel extracts by cytolase enhance antioxidant and anti-inflammatory capacity in RAW 264.7 cells / Nutrition Research and Practice / 한국영양학회 10(2) : 131~138

[journal] Ciesielska, A / 2021 / TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling / Cellular and molecular life sciences 78 : 1233~1261

[journal] CHEN, R. M. / 2005 / Anti‐inflammatory and antioxidative effects of propofol on lipopolysaccharide‐activated macrophages / Annals of the New York Academy of Sciences 1042 : 262~271

[journal] Liang, Y.-C. / 1999 / Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages / Carcinogenesis 20 : 1945~1952

[journal] Hotamisligil, G. S. / 2017 / Inflammation, metaflammation and immunometabolic disorders / Nature 542 : 177~185

[journal] Cobb, M. H. / 1995 / How MAP Kinases Are Regulated (∗) / Journal of Biological Chemistry 270 : 14843~14846

[journal] Dumitru, C. D / 2000 / TNF-α induction by LPS is regulated posttranscriptionally via a Tpl2/ERK-dependent pathway / Cell 103 : 1071~1083

[journal] Ghosh, S / 1998 / NF-κB and Rel proteins: evolutionarily conserved mediators of immune responses / Annual review of immunology 16 : 225~260

[journal] Choi, K.-C. / 2016 / Intestinal anti-inflammatory activity of the seeds of Raphanus sativus L. in experimental ulcerative colitis models / Journal of ethnopharmacology 179 : 55~65

[journal] Baek, S.-H / 2008 / Protective effects of an extract of young radish (Raphanus sativus L) cultivated with sulfur (sulfur-radish extract) and of sulforaphane on carbon tetrachloride-induced hepatotoxicity / Bioscience, biotechnology, and biochemistry 72 : 1176~1182

[journal] Kook, S.-H / 2014 / Raphanus sativus L. seeds prevent LPS-stimulated inflammatory response through negative regulation of the p38 MAPK-NF-κB pathway / International immunopharmacology 23 : 726~734

[journal] Wongrakpanich, S / 2018 / A comprehensive review of non-steroidal anti-inflammatory drug use in the elderly / Aging and disease 9 : 143~

[journal] Khamees, A. H. / 2017 / Phytochemical and pharmacological analysis for seeds of two varieties of Iraqi Raphanus sativus / Int J Pharm Sci Rev Res 43 : 237~242

[journal] Salis, K. M. / 2015 / Beneficial effects of vegetable oils (Rice bran and Mustard oils) on anti-inflammatory and gastro intestinal profiles of indomethacin in rats / Biomedical and Pharmacology Journal 6 : 375~379

[journal] Facchin, B. M. / 2022 / Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a systematic review and meta-analysis / Inflammation Research 71 : 741~758

[journal] Kaminska, B. / 2005 / MAPK signalling pathways as molecular targets for anti-inflammatory therapy—from molecular mechanisms to therapeutic benefits / Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1754 : 253~262

KJCKorea
Journal Central

CELLMED 2023 KCI Impact Factor : 0.02

Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS

ABSTRACT

KEYWORDS

Citation status

* References for papers published after 2023 are currently being built.

CELLMED 2023 KCI Impact Factor : 0.02

Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS

ABSTRACT

KEYWORDS

Statistics

Tools

Issue List

Citation status

KCI Citation Counts (0)

REFERENCES (16) * References for papers published after 2023 are currently being built.

Search PDF

Citation

* References for papers published after 2023 are currently being built.