@article{ART002961730},
author={Bong-Keun Jang and Sunyoung Park and Dahyun Mun and Gunwoo Lee and Youngsun Kwon and Hye-yeon Kang and Jeom-Yong Kim},
title={Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS},
journal={CELLMED},
issn={3022-6805},
year={2023},
volume={13},
number={6},
pages={7-7},
doi={10.5667/CellMed.2023.007}
TY - JOUR
AU - Bong-Keun Jang
AU - Sunyoung Park
AU - Dahyun Mun
AU - Gunwoo Lee
AU - Youngsun Kwon
AU - Hye-yeon Kang
AU - Jeom-Yong Kim
TI - Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS
JO - CELLMED
PY - 2023
VL - 13
IS - 6
PB - Cellmed Orthocellular Medicine and Pharmaceutical Association
SP - 7
EP - 7
SN - 3022-6805
AB - Raphanus sativus L. has been reported to have anti-inflammatory and anti-tumor activity. However, the anti-inflammatory effect and mechanism of action of the Raphanus sativus L. seeds (RSS) with or without oil are still unknown. This study was undertaken to investigate the in-vitro anti-inflammatory effect with or without oil in the RSS on RAW 264.7 cells stimulated by lipopolysaccharide (LPS). Results showed the suppressed LPS-induced secretion of pro-inflammatory mediators such as nitric oxide (NO), inflammatory cytokine (IL-6, TNF-α). Additionally, a decrease in protein expression of iNOS was observed, but nuclear translocation of NF-κB p65 was not inhibited. To elucidate the underlying mechanism of the anti-inflammatory effect of RSS, the involvement of mitogen-activated protein kinase (MAPK) signaling pathways was examined. We also found that RSS blocked LPS-induced phosphorylation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK) signaling but did not affect the phosphorylation of p38 MAPK and extracellular signal-regulated kinase (ERK) 1/2. These results suggest that RSS may have potential as an anti-inflammatory agent through the inhibition of LPS-induced inflammatory cytokine production via regulation of the JNK pathway.
KW - Raphanus sativus L. seed;anti-inflammation;macrophage;NO
DO - 10.5667/CellMed.2023.007
ER -
Bong-Keun Jang, Sunyoung Park, Dahyun Mun, Gunwoo Lee, Youngsun Kwon, Hye-yeon Kang and Jeom-Yong Kim. (2023). Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS. CELLMED, 13(6), 7-7.
Bong-Keun Jang, Sunyoung Park, Dahyun Mun, Gunwoo Lee, Youngsun Kwon, Hye-yeon Kang and Jeom-Yong Kim. 2023, "Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS", CELLMED, vol.13, no.6 pp.7-7. Available from: doi:10.5667/CellMed.2023.007
Bong-Keun Jang, Sunyoung Park, Dahyun Mun, Gunwoo Lee, Youngsun Kwon, Hye-yeon Kang, Jeom-Yong Kim "Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS" CELLMED 13.6 pp.7-7 (2023) : 7.
Bong-Keun Jang, Sunyoung Park, Dahyun Mun, Gunwoo Lee, Youngsun Kwon, Hye-yeon Kang, Jeom-Yong Kim. Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS. 2023; 13(6), 7-7. Available from: doi:10.5667/CellMed.2023.007
Bong-Keun Jang, Sunyoung Park, Dahyun Mun, Gunwoo Lee, Youngsun Kwon, Hye-yeon Kang and Jeom-Yong Kim. "Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS" CELLMED 13, no.6 (2023) : 7-7.doi: 10.5667/CellMed.2023.007
Bong-Keun Jang; Sunyoung Park; Dahyun Mun; Gunwoo Lee; Youngsun Kwon; Hye-yeon Kang; Jeom-Yong Kim. Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS. CELLMED, 13(6), 7-7. doi: 10.5667/CellMed.2023.007
Bong-Keun Jang; Sunyoung Park; Dahyun Mun; Gunwoo Lee; Youngsun Kwon; Hye-yeon Kang; Jeom-Yong Kim. Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS. CELLMED. 2023; 13(6) 7-7. doi: 10.5667/CellMed.2023.007
Bong-Keun Jang, Sunyoung Park, Dahyun Mun, Gunwoo Lee, Youngsun Kwon, Hye-yeon Kang, Jeom-Yong Kim. Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS. 2023; 13(6), 7-7. Available from: doi:10.5667/CellMed.2023.007
Bong-Keun Jang, Sunyoung Park, Dahyun Mun, Gunwoo Lee, Youngsun Kwon, Hye-yeon Kang and Jeom-Yong Kim. "Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS" CELLMED 13, no.6 (2023) : 7-7.doi: 10.5667/CellMed.2023.007