Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS

Bong-Keun Jang (장봉근); Sunyoung Park (박선영); Dahyun Mun (문다현); Gunwoo Lee (이건우); Youngsun Kwon (권영선); Hye-yeon Kang (강혜연); Jeom-Yong Kim (김점용)

doi:10.5667/CellMed.2023.007

Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS

CELLMED
Abbr : CellMed
2023, 13(6), pp.7-7
DOI : 10.5667/CellMed.2023.007
Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
Research Area : Medicine and Pharmacy > General Medicine
Received : May 11, 2023
Accepted : May 19, 2023
Published : May 31, 2023

Bong-Keun Jang ¹, Sunyoung Park ¹, Dahyun Mun ¹, Gunwoo Lee ¹, Youngsun Kwon ¹, Hye-yeon Kang ¹, Jeom-Yong Kim ¹

¹JBK-LAB, Inc.

Accredited

ABSTRACT

Raphanus sativus L. has been reported to have anti-inflammatory and anti-tumor activity. However, the anti-inflammatory effect and mechanism of action of the Raphanus sativus L. seeds (RSS) with or without oil are still unknown. This study was undertaken to investigate the in-vitro anti-inflammatory effect with or without oil in the RSS on RAW 264.7 cells stimulated by lipopolysaccharide (LPS). Results showed the suppressed LPS-induced secretion of pro-inflammatory mediators such as nitric oxide (NO), inflammatory cytokine (IL-6, TNF-α). Additionally, a decrease in protein expression of iNOS was observed, but nuclear translocation of NF-κB p65 was not inhibited. To elucidate the underlying mechanism of the anti-inflammatory effect of RSS, the involvement of mitogen-activated protein kinase (MAPK) signaling pathways was examined. We also found that RSS blocked LPS-induced phosphorylation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK) signaling but did not affect the phosphorylation of p38 MAPK and extracellular signal-regulated kinase (ERK) 1/2. These results suggest that RSS may have potential as an anti-inflammatory agent through the inhibition of LPS-induced inflammatory cytokine production via regulation of the JNK pathway.

KEYWORDS

Raphanus sativus L. seed, anti-inflammation, macrophage, NO

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CELLMED 2023 KCI Impact Factor : 0.03

Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS

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* References for papers published after 2023 are currently being built.

CELLMED 2023 KCI Impact Factor : 0.03

Comparison of Anti-Inflammation Effects of Specimens Before and After the Oil Extraction of Raphanus sativus L. Seed in RAW 264.7 Macrophage Activated by LPS

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