@article{ART003139052},
author={Bong-Keun Jang and Hye-yeon Kang and Sunyoung Park and Gunwoo Lee and Youngsun Kwon and Sang-Eun Shin},
title={Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria},
journal={CELLMED},
issn={3022-6805},
year={2024},
volume={14},
number={14},
pages={1-7}
TY - JOUR
AU - Bong-Keun Jang
AU - Hye-yeon Kang
AU - Sunyoung Park
AU - Gunwoo Lee
AU - Youngsun Kwon
AU - Sang-Eun Shin
TI - Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria
JO - CELLMED
PY - 2024
VL - 14
IS - 14
PB - Cellmed Orthocellular Medicine and Pharmaceutical Association
SP - 1
EP - 7
SN - 3022-6805
AB - Influenza is an infectious disease which accompanies systematic inflammation. Although eliminating the primary or secondary infectious agent is an important action in treating influenza, it is still difficult because not only mutations of viruses but also antibiotics-resistance of bacteria. Eunkyosan, is traditionally used herbal formula to symptoms of influenza, have were reported for its pharmacological properties on inflammation. Also, Alginate, a physiologically active acidic polysaccharide obtained from brown algae, alleviates inflammation, exhibits anti-bacterial properties, and shows a collaborative therapeutic effect with an anti-bacterial agent for acute infections. Thus, we investigated anti-inflammatory effects of Lorafocin (a mixture of Eunkyosan and alginate) against lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophage cell line and its underlying mechanisms as well as anti-bacterial effects. In the RAW 264.7 macrophage cell line, Lorafocin treatment reduced the secretion of inflammatory mediators such as nitric oxide (NO) and pro-inflammatory cytokines induced by LPS. To elucidate the anti-inflammatory mechanism of Lorafocin, we investigated the involvement of the mitogen-activated protein kinase (MAPK) and protein kinase B (Akt) signaling pathway. Studies demonstrated that Lorafocin exhibited anti-inflammatory efficacy by regulating Akt, c-Jun N-terminal kinase/stress-activated protein kinase (JNK), and extracellular signal-regulated kinase (ERK) 1/2 in LPS-induced RAW264.7 macrophages. Furthermore, Lorafocin inhibited the bacterial growth of Staphylococcus aureus and Escherichia coli. Taken together, Lorafocin may possess the capability to effectively modulate inflammatory responses and inhibit the growth of pathogenic bacteria.
KW - Lorafocin;Eunkyosan;Alginate;Anti-inflammation;Anti-bacteria
DO -
UR -
ER -
Bong-Keun Jang, Hye-yeon Kang, Sunyoung Park, Gunwoo Lee, Youngsun Kwon and Sang-Eun Shin. (2024). Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria. CELLMED, 14(14), 1-7.
Bong-Keun Jang, Hye-yeon Kang, Sunyoung Park, Gunwoo Lee, Youngsun Kwon and Sang-Eun Shin. 2024, "Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria", CELLMED, vol.14, no.14 pp.1-7.
Bong-Keun Jang, Hye-yeon Kang, Sunyoung Park, Gunwoo Lee, Youngsun Kwon, Sang-Eun Shin "Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria" CELLMED 14.14 pp.1-7 (2024) : 1.
Bong-Keun Jang, Hye-yeon Kang, Sunyoung Park, Gunwoo Lee, Youngsun Kwon, Sang-Eun Shin. Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria. 2024; 14(14), 1-7.
Bong-Keun Jang, Hye-yeon Kang, Sunyoung Park, Gunwoo Lee, Youngsun Kwon and Sang-Eun Shin. "Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria" CELLMED 14, no.14 (2024) : 1-7.
Bong-Keun Jang; Hye-yeon Kang; Sunyoung Park; Gunwoo Lee; Youngsun Kwon; Sang-Eun Shin. Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria. CELLMED, 14(14), 1-7.
Bong-Keun Jang; Hye-yeon Kang; Sunyoung Park; Gunwoo Lee; Youngsun Kwon; Sang-Eun Shin. Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria. CELLMED. 2024; 14(14) 1-7.
Bong-Keun Jang, Hye-yeon Kang, Sunyoung Park, Gunwoo Lee, Youngsun Kwon, Sang-Eun Shin. Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria. 2024; 14(14), 1-7.
Bong-Keun Jang, Hye-yeon Kang, Sunyoung Park, Gunwoo Lee, Youngsun Kwon and Sang-Eun Shin. "Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria" CELLMED 14, no.14 (2024) : 1-7.
