Neuroprotective Effects of Nebbiolo Red Wine Against Ethanol-induced Cognitive Dysfunction and Neurotoxicity

Jong Ae Choi (최종애); Seong-Hoon Yun (윤성훈); Hye-Yeon Kang (강혜연); Sunyoung Park (박선영); Sung-Bae Lee (이성배); Gun-Woo Lee (이건우); Young-Sun Kwon (권영선); Hee-Yeong Jeong (정희영); Sang-Eun Shin (신상은); Na Rae Park (박나래); Bong-Keun Jang (장봉근); Kwang-soon Shin (신광순)

doi:10.5667/CellMed.2025.015

Neuroprotective Effects of Nebbiolo Red Wine Against Ethanol-induced Cognitive Dysfunction and Neurotoxicity

CELLMED
Abbr : CellMed
2025, 15(14), e15
DOI : 10.5667/CellMed.2025.015
Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
Research Area : Medicine and Pharmacy > General Medicine
Received : November 24, 2025
Accepted : November 28, 2025
Published : November 28, 2025

Jong Ae Choi ¹, Seong-Hoon Yun ², Hye-Yeon Kang ², Sunyoung Park ², Sung-Bae Lee ², Gun-Woo Lee ², Young-Sun Kwon ², Hee-Yeong Jeong ², Sang-Eun Shin ², Na Rae Park ², Bong-Keun Jang ², Kwang-soon Shin ³

¹VINO VISTA
²JBK-LAB, Inc.
³경기대학교

Accredited

ABSTRACT

Objective: Chronic alcohol consumption causes neurotoxicity, yet moderate red wine consumption is linked to health benefits, often attributed to its polyphenols. This study investigated whether Nebbiolo red wine (NRW), a variety with high polyphenol content, possesses sufficient neuroprotective capacity to counteract the concurrent neurotoxic effects of its own ethanol (EtOH) content. Materials and Methods: Neuroprotective effects were evaluated in vitro (SH-SY5Y cells, WST-8 assay) and in vivo (mice, Y-maze test) following exposure to 14% EtOH or 14% NRW. Antioxidant capacity was determined by DPPH and ORAC assays. Results: NRW demonstrated robust dose-dependent antioxidant activity. In vitro, NRWP significantly protected SH-SY5Y cells from EtOH-induced cytotoxicity. In vivo, 14% EtOH administration severely impaired spatial memory (27.1% alternation vs. 67.5% Sham), an effect completely prevented by 14% NRW (65% alternation). Conclusion: NRW mediates significant neuroprotection, effectively neutralizing ethanol-induced cytotoxicity in vitro and cognitive impairment in vivo. This suggests polyphenolic content is a critical determinant of alcohol's net neurological impact.

KEYWORDS

Nebbiolo red wine, Ethanol, Neuroprotection, Oxidative Stress, Cognitive Impairment, Y-maze

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[journal] Deví-Bastida J / 2024 / Relationship between alcohol consumption and cognitive impairment in the adult population over 60 years of age: a systematic review / Rev Colomb Psiquiatr (Engl Ed) 53(3) : 385~395

[journal] Nunes PT / 2019 / Aging with alcohol-related brain damage: Critical brain circuits associated with cognitive dysfunction / Int Rev Neurobiol 148 : 101~168

[journal] Rehm J / 2019 / Alcohol use and dementia: a systematic scoping review / Alzheimers Res Ther 11(1) : 1~

[journal] Wang G / 2023 / Alcohol Use Disorder as a Risk Factor for Cognitive Impairment / J Alzheimers Dis 94(3) : 899~907

[journal] Tiwari V / 2012 / Neuroprotective effect of vitamin E isoforms against chronic alcohol-induced peripheral neurotoxicity: possible involvement of oxidative-nitrodative stress / Phytother Res. 26(11) : 1738~1745

[journal] Haorah J / 2008 / Mechanism of alcohol-induced oxidative stress and neuronal injury / Free Radic Biol Med 45(11) : 1542~1550

[journal] Tsermpini EE / 2022 / Alcohol-induced oxidative stress and the role of antioxidants in alcohol use disorder: a systematic review / Antioxidants (Basel) 11(7) : 1374~

[journal] Erickson EK / 2019 / Neuroimmune signaling in alcohol use disorder / Pharmacol Biochem Behav : 34~60

[journal] Adams C / 2023 / Central markers of neuroinflammation in alcohol use disorder: A meta-analysis of neuroimaging, cerebral spinal fluid, and postmortem studies / Alcohol Clin Exp Res 47(4) : 533~557

[journal] Mitoma H / 2021 / Mechanisms of Ethanol-Induced Cerebellar Ataxia: Underpinnings of Neuronal Death in the Cerebellum / Int J Environ Res Public Health 18(16) : 8678~

[confproc] Du S / 2019 / The Neurotoxicity of Ethanol and The Underlining Mechanisms / IOP Conference Series: Materials Science and Engineering 612(2) : 022028~

[journal] Basli A / 2012 / Wine polyphenols: potential agents in neuroprotection / Oxid Med Cell Longev : 805762~

[journal] Buljeta I / 2023 / Beneficial Effects of Red Wine Polyphenols on Human Health: Comprehensive Review / Curr Issues Mol Biol 45(2) : 782~798

[journal] Rice-Evans CA / 1996 / Structure-antioxidant activity relationships of flavonoids and phenolic acids / Free Radic Biol Med 20(7) : 933~956

[journal] Leopoldini M / 2011 / The molecular basis of working mechanism of natural polyphenolic antioxidants / Food Chem. 125(2) : 288~306

[journal] Jomova K / 2024 / Flavonoids and their role in oxidative stress, inflammation, and human diseases / J Agric Food Chem 72(15) : 7399~7428

[journal] Azevedo J / 2020 / Polyphenolic Characterization of Nebbiolo Red Wines and Their Interaction with Salivary Proteins / Foods 9(12) : 1867~

[journal] Piombino P / 2020 / Preliminary sensory characterisation of the diverse astringency of single cultivar Italian red wines and correlation of sub-qualities with chemical composition / Aust J Grape Wine Res 26(3) : 283~296

[other] U.S. Food and Drug Administration / 2005 / Guidance for Industry: Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers

[journal] Wang J / 2006 / Moderate consumption of Cabernet Sauvignon attenuates Aβ neuropathology in a mouse model of Alzheimer's disease / FASEB J. 20(13) : 2313~2320

[journal] Leopoldini M / 2011 / The molecular basis of working mechanism of natural polyphenolic antioxidants / Food Chem. 125(2) : 288~306

[journal] Assunção M / 2007 / Red wine antioxidants protect hippocampal neurons against ethanol-induced damage: a new mechanism of action? / Folia Neuropathol. 45(3) : 115~123

[journal] Vauzour D. / 2012 / Dietary polyphenols as modulators of brain functions: biological actions and molecular mechanisms underpinning their beneficial effects / Oxid Med Cell Longev : 914273~

[journal] Yuan H / 2013 / Neuroprotective effects of resveratrol on embryonic dorsal root ganglion neurons with neurotoxicity induced by ethanol / Food Chem Toxicol 55 : 192~201

[journal] Zięba A / 2025 / Neuroprotective effects of wine polyphenols in Alzheimer's and Parkinson's diseases: a review of risks and benefits / Beverages (Basel) 11(5) : 131~

[journal] Pandey SC / 2016 / A Critical Role of Brain-Derived Neurotrophic Factor in Alcohol Consumption / Biol Psychiatry 79(6) : 427~429

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CELLMED 2025 KCI Impact Factor : 0.18

Neuroprotective Effects of Nebbiolo Red Wine Against Ethanol-induced Cognitive Dysfunction and Neurotoxicity

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CELLMED 2025 KCI Impact Factor : 0.18

Neuroprotective Effects of Nebbiolo Red Wine Against Ethanol-induced Cognitive Dysfunction and Neurotoxicity

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