2014, Vol.41, No.4

Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

pISSN : 1226-8496 / eISSN : 2288-3819
https://journal.kci.go.kr/jkapd

Patients with extreme anxiety who are unable to cope with dental treatment under non-pharmacologicalbehavioral management method may require sedation, or other forms pharmacological behavioral managementmethod. The aim of this retrospective study was to investigate the effect of concomitant administration of 0.1mg/kg intramuscular midazolam with or without sevoflurane insufflation sedation on sedation depth andcardiopulmonary function in pediatric patients. We analysed the records of anesthesia on patients who receiveddental treatment under deep sedation using sevoflurane insufflation from January 2013 to March 2014. Thirtysixchildren, aged 3 to 6 years, undergoing dental treatment were sedated using either sevoflurane insufflationalone (Group S, n = 18) or a combination of intramuscular injection of 0.1 mg/kg midazolam plus sevofluraneinsufflation (Group SM, n = 18). Upon comparison, the average entropy value of group SM was lower than thatof group S, but there were no statistically significant difference between the two groups (p > 0.05). The averageheart rate and mean arterial pressure of group SM were higher than those of group S (p < 0.05). Concomitantintramuscular injection of 0.1 mg/kg midazolam with sevoflurane insufflation sedation is not sufficient to enhancethe quality of sedation.

DNA extraction is a prerequisite for the identification of pathogens in clinical samples. Commercial DNA extractionkits generally involve time-consuming and laborious multi-step procedures. In the present study, our modified DNA isolation method for saliva samples allows for the quick detection ofpathogens associated with dental caries or periodontitis by PCR within 1 h. To release DNA from the bacteria, 1min of boiling was adequate, and the resulting isolated DNA can be used many times and is suitable for longterm storage of at least 13 months at 4℃, and even longer at -20℃. In conclusion, our modified DNA extraction method is simple, rapid, and cost-effective, and suitable forpreparing DNA from clinical samples for PCR for the rapid detection of oral pathogens from saliva.

The purpose of this study is to evaluate the effects of facemask therapy in patients with Class III malocclusionwith two types of bonded expanders covering different numbers of anchored teeth and to compare the anchoragevalue of two types of bonded expander. Eighteen subjects with Class III malocclusion in early mixed dentition were included in this study, andsubjects were divided into two groups based on the number of teeth covered by bonded expander: group 1(splinting four teeth on each side, 9 subjects) and group 2 (splinting three teeth on each side, 9 subjects). Lateral cephalograms were obtained and assessed before (T1) and after (T2) the treatment. The facemask therapy showed skeletal effects including anterior movement of maxilla and backward rotationof mandible in both groups, with no significant differences between groups. Mesial movement of maxillarymolars which indicates anchorage loss of the bonded expander was found in both groups, but significantly largermesial movement was found in group 2 than in group 1. In conclusion, the value of anchorage was different according to the number of teeth covered by bondedexpander as an intraoral anchorage of facemask, but there were no significant differences in skeletal effects.