Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method

Jaehwan Kim (김재환); 김미아; Daewoo Lee (이대우); Byeong Ju Baik (백병주); Yang Yeon Mi (양연미); KIM JAE GON (김재곤)

doi:10.5933/JKAPD.2014.41.4.292

Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2014, 41(4), pp.292-297
DOI : 10.5933/JKAPD.2014.41.4.292
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry

Jaehwan Kim ¹, 김미아 ², Daewoo Lee ¹, Byeong Ju Baik ³, Yang Yeon Mi ³, KIM JAE GON ³

¹전북대학교 치의학전문대학원 소아치과학교실 및 구강생체과학연구소
²전북대학교 치과대학 소아치과학교실 및 구강생체과학연구소
³전북대학교

Accredited

ABSTRACT

DNA extraction is a prerequisite for the identification of pathogens in clinical samples. Commercial DNA extractionkits generally involve time-consuming and laborious multi-step procedures. In the present study, our modified DNA isolation method for saliva samples allows for the quick detection ofpathogens associated with dental caries or periodontitis by PCR within 1 h. To release DNA from the bacteria, 1min of boiling was adequate, and the resulting isolated DNA can be used many times and is suitable for longterm storage of at least 13 months at 4℃, and even longer at -20℃. In conclusion, our modified DNA extraction method is simple, rapid, and cost-effective, and suitable forpreparing DNA from clinical samples for PCR for the rapid detection of oral pathogens from saliva.

KEYWORDS

DNA extraction, Oral pathogens, Saliva, Polymerase chain reaction(PCR)

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This paper was written with support from the National Research Foundation of Korea.

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Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method

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* References for papers published after 2023 are currently being built.

Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method

ABSTRACT

KEYWORDS

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