2012, Vol.22, No.1

Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6

pISSN : 1229-1854
https://journal.kci.go.kr/jkmr

Objectives :The aim of this study was to investigate the acute toxicity and safety of fermented deer velvet antler extract in institute for cancer research(ICR) mice. Methods : To evaluate the acute toxicity and safety, fermented deer antler at the respective doses of 0(control group), 2560, 3200, 4000 and 5000 mg/kg were given orally to 25 male ICR mice for 14 days and allocated 5 mice for each group. After a single administration of fermented deer antler extract, we observed survival rates, general toxicity and any changes in body weight. Autopsies were conducted on all 25 mice. Results : Compared with the control group, we did not find any toxic alterations in any of the treated groups. Conclusions : A single dose of 5000 mg/kg of fermented deer antler extract was found to be safe in ICR mice. However, anything beyond this level has the potential to be lethal.

Objectives :The aim of this study was to investigate the Anti-muscle fatigue effect of fermented deer velvet antler extract in Sprague-Dawley rats. Methods : Rats were randomly divided into four groups of 10 mice each according to a randomized block design. Saline solution was orally administered to the control group, 100 mg/kg of water-extract of deer velvet antler to the positive control group, 50 mg/kg of fermented deer velvet antler extract to experimental group 1 and 100 mg/kg of fermented deer velvet antler extract to experimental group 2. Each of the four groups were treated for seven days and then participated in a forced swimming test which applied both a high intensity exercise model and a prolonged exercise model. We observed swimming times and examined blood biochemical parameters. Results : In the high intensity exercise model, the effect of the deer velvet antler was seen in the increased swimming times. Experimental group 2 swam for approximately 600% longer as compared to the control group(p<0.001), indicating that the recovery effect is dose-dependent. In the forced swimming tests using the prolonged exercise model, blood sugar levels(p<0.001) and free fatty acid levels(p<0.05, p<0.01 each) in the positive control group and experimental groups 1 and 2 demonstrated a significant recovery effect in comparison with the control group. Conclusions : Fermented deer velvet antler extract may have an Anti-muscle fatigue effect similar to that of deer velvet antler extract and could be used when it has an effect equal to one-fifth of the amount considering the yield rate.

Objectives :The object of this study is to observe the favorable anti-arthritic effects of Imyo-san(Èrmiào-sǎn)(IMS), that has been traditionally used in Korean medicine to treat rheumatoid arthritis, on Freund’s complete adjuvant(FCA)-induced arthritic Wistar rats. Methods :Rheumatoid arthritis was induced by intradermal injection of FCA(10 mg in 1 ml paraffin oil 0.1 ml/rats). Each of 8 rats showing regular ankle thicknesses, per group selected after 14 days after FCA treatment to confirm the induction of rheumatoid arthritis. 300, 150 or 750 mg/kg of IMS were orally administered once a day for 14 days from 14 days after FCA treatments. Dexamethasone was intraperitoneally administered 15 mg/kg, once a day for 14 days from 14 days after FCA treatments. Experimental animals were sacrificed after 14 days of continuous oral treatment of IMS or intraperitoneal administration of dexamethasone, and changes on the body weight, knee circumferences, gross arthritis score, inflammatory tissue prostaglandin(PG) E2 level sand cartilage collagen, glucosaminoglycans compositions–chondroitin sulphate, heparan sulphate and hyaluronic acid in the present study. Results :As results of FCA treatment, classic rheumatoid arthritis featuring dramatical decreases on the body weight, cartilage collagen contents and bone glucosaminoglycans - chondroitin sulphate, heparan sulphate and hyaluronic acid contents, with increases on the knee circumferences, gross arthritis scores and inflammatory tissue PGE2 levels. However, these changes from FCA-induced rheumatoid arthritis were clearly reduced by treatment of dexamethasone and both two different dosages of IMS, 300 and 150 mg/kg in the present study. Although FCA-induced arthritis were more favorably inhibited by treatment of dexamethasone 15 mg/kg as compared with IMS 300 mg/kg, marked decreases of body weights were detected in dexamethasone 15 mg/kg treated rats. Conclusions :The results obtained in this study suggest that over 150 mg/kg of IMS showed favorable anti-arthritic effects on the FCA-induced arthritis mediated by suppression of PGE2. However, detailed mechanism studies should be carried out in future with the screening of the biological active compounds in this herb. Although FCA-induced arthritis were more favorably inhibited by treatment of dexamethasone 15 mg/kg as compared with IMS 300 mg/kg, marked decreases of body weights were detected in dexamethasone 15 mg/kg treated rats, in the present study.