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Effect of Unfermented and Fermented Atractylodes macrocephalae on Gut Permeability and Lipopolysaccharide-Induced Inflammation

  • Journal of Korean Medicine for Obesity Research
  • Abbr : J Korean Med Obes Res
  • 2013, 13(1), pp.24-32
  • Publisher : The Society of Korean Medicine for Obesity Research
  • Research Area : Medicine and Pharmacy > Korean Medicine

Kyungsun Han 1,  김기철 2,  Jing-Hua Wang 2,  Hojun Kim 2

1동국대학교 일산한방병원 한방재활의학과
2동국대학교일산한방병원 한방재활의학과

ABSTRACT

Objectives: The aim of this study is to investigate anti-imflammatory and protective effect forintestinal epithelial cells with Atractylodes macrocephae (AM), a traditional Korean Herbalmedicine and fermented Atractylodes macrocephae (FAM) with Lactobacillus plantarum. Methods: HCT-116 and Raw 264.7 cells were used in this study. Using NO assay, we measuredlipopolysaccharide (LPS)-induced anti-inflammatory effect. We measured permeability ofintestinal epithelial cells with transepithelial electrical resistance and horseradish peroxide fluxassay. Water soluble tetrazolium salt assay was used to see cell proliferation. All the results werepresented in mean and standard deviation. We used Student's t-test for analyzing significance ofresults. Results: In Raw 264.7 cells NO production decreased 22.4% with pre-treatment of AM and FAM,especially with FAM in high concentration. In HCT-116 cells LPS-induced intestinal permeabilityhad a protective effect with both AM and FAM, which was also tend to be proportional to theconcentration. Cell viability increased up to 135.52% after treatment of high concentration ofFAM in HCT-116, while there was no significant change in Raw 264.7 cells with herb treatments. Conclusions: These results show evidence that AM, especially fermented ones, significantly reduced intestinal membrance permeability. They also had a protective effect as well as an anti-inflammation effect for HCT-116 and Raw 264.7 cells. This suggest that FAM may be a therapeutic agent for Leaky gut syndrome by reducing intestinal permeability.

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