@article{ART002667360},
author={Hye Min Choi and Byung-Woo Yu and Min-Ju Kim and Jung-Ok Kim},
title={Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions},
journal={Journal of Korean Medicine for Obesity Research},
issn={1976-9334},
year={2020},
volume={20},
number={2},
pages={78-87},
doi={10.15429/jkomor.2020.20.2.78}
TY - JOUR
AU - Hye Min Choi
AU - Byung-Woo Yu
AU - Min-Ju Kim
AU - Jung-Ok Kim
TI - Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions
JO - Journal of Korean Medicine for Obesity Research
PY - 2020
VL - 20
IS - 2
PB - The Society of Korean Medicine for Obesity Research
SP - 78
EP - 87
SN - 1976-9334
AB - Objectives: To expand and provide information on the efficacy of herbal medicines, anti-obesity effects were evaluated. In many studies, plant-derived components with anti-obesity efficacies have been investigated for their potential inhibitory effects on 3T3-L1 preadipocyte cells. The purpose of this study was to investigate the anti-obesity effects of 65 herbal medicine in 3T3-L1 preadipocyte cells.
Methods: Preferentially, 3T3-L1 cells were treated with 65 herbal medicines (500 µg/mL) during differentiation for 8 days. Next, 3T3-L1 cells were treated with selected herbal medicines at concentrations ranging from 50 to 200 µg/mL during differentiation for 8 days. The accumulation of lipid droplets was determined by Oil Red O staining. The expressions of genes related to adipogenesis were measured by reverse transcription polymerase chain reaction and Western blot analyses.
Results: Among the 65 kinds of herbal medicines, 13 herbal medicines that been shown to be effective against the accumulation of lipid droplets were selected. Finally, selected Banhasasim-tang and Samhwangsasim-tang showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affecting cell toxicity. In addition, Banhasasim-tang and Samhwangsasim-tang significantly reduced the expression levels of several adipocyte marker genes including peroxisome proliferator activated receptor-γ and CCAAT/enhancer binding protein-α.
Conclusions : These results suggest that the ability of Banhasasim-tang and Samhwangsasimtang has inhibited overall adipogenesis and lipid accumulation in the 3T3-L1 cells.
Banhasasim-tang and Samhwangsasim-tang may be a promising medicine for the treatment of obesity and related metabolic disorders.
KW - Herbal medicine;obesity;3T3-L1
DO - 10.15429/jkomor.2020.20.2.78
ER -
Hye Min Choi, Byung-Woo Yu, Min-Ju Kim and Jung-Ok Kim. (2020). Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions. Journal of Korean Medicine for Obesity Research, 20(2), 78-87.
Hye Min Choi, Byung-Woo Yu, Min-Ju Kim and Jung-Ok Kim. 2020, "Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions", Journal of Korean Medicine for Obesity Research, vol.20, no.2 pp.78-87. Available from: doi:10.15429/jkomor.2020.20.2.78
Hye Min Choi, Byung-Woo Yu, Min-Ju Kim, Jung-Ok Kim "Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions" Journal of Korean Medicine for Obesity Research 20.2 pp.78-87 (2020) : 78.
Hye Min Choi, Byung-Woo Yu, Min-Ju Kim, Jung-Ok Kim. Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions. 2020; 20(2), 78-87. Available from: doi:10.15429/jkomor.2020.20.2.78
Hye Min Choi, Byung-Woo Yu, Min-Ju Kim and Jung-Ok Kim. "Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions" Journal of Korean Medicine for Obesity Research 20, no.2 (2020) : 78-87.doi: 10.15429/jkomor.2020.20.2.78
Hye Min Choi; Byung-Woo Yu; Min-Ju Kim; Jung-Ok Kim. Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions. Journal of Korean Medicine for Obesity Research, 20(2), 78-87. doi: 10.15429/jkomor.2020.20.2.78
Hye Min Choi; Byung-Woo Yu; Min-Ju Kim; Jung-Ok Kim. Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions. Journal of Korean Medicine for Obesity Research. 2020; 20(2) 78-87. doi: 10.15429/jkomor.2020.20.2.78
Hye Min Choi, Byung-Woo Yu, Min-Ju Kim, Jung-Ok Kim. Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions. 2020; 20(2), 78-87. Available from: doi:10.15429/jkomor.2020.20.2.78
Hye Min Choi, Byung-Woo Yu, Min-Ju Kim and Jung-Ok Kim. "Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions" Journal of Korean Medicine for Obesity Research 20, no.2 (2020) : 78-87.doi: 10.15429/jkomor.2020.20.2.78