@article{ART001677481},
author={지윤정},
title={Toothbrush sterilizing effects of using microwave},
journal={Journal of Korean society of Dental Hygiene},
issn={2287-1705},
year={2012},
volume={12},
number={3},
pages={641-646},
doi={}
TY - JOUR
AU - 지윤정
TI - Toothbrush sterilizing effects of using microwave
JO - Journal of Korean society of Dental Hygiene
PY - 2012
VL - 12
IS - 3
PB - Korean Society of Dental Hygiene
SP - 641
EP - 646
SN - 2287-1705
AB - Objectives : The purpose of this study is to analyze the sterilizing effects of toothbrushes by administering bacteria into toothbrushes and reproducing the antibacterial effects using a microwave oven.
Methods : The heads of four-row mid-strength toothbrushes were cut, put in a bacterial solution (3×109cells/ml) for vortexing, and sterilized with microwaves for 0, 30, and 60 seconds. They were then moved into four tubes containing DW 10 ml and suspended in a vortex mixer for two minutes to separate bacteria from them. DW 9ml was added by 1ml of bacteria for dilution of 10~106 times. It was then administered to the BHI agar plate by 0.1ml and cultured at 37℃ for 24 hours. Total number of bacteria adhered to a toothbrush was obtained by multiplying the number of colonies by the dilution factor. The experiment was done in the first, second, and third step, being repeated in a normal temperature drier (23℃) after 5, 9 and 24 hours.
Results : The results of the experiment revealed that the sterilizing effects were 95% or over. When toothbrushes were sterilized for 60 seconds, the number of colonies is about 11 after drying for 5 hours, 7after drying for 9 hours and 2 after drying for 24 hours.
The sterilizing effects reached 98% when the bacteria-administered toothbrush was sterilized for 1 minute after drying for 24 hours.
Conclusions : The results demonstrated that toothbrush sterilizing by using microwave is a suitable way to prevent cross-contamination of toothbrushes by oral bacterial infection and thus easy to use at home.
However, this study suggests that toothbrush sterilizing by using microwave should be limited within two times a week because the physical properties of toothbrush might be changed.
KW - microwave;sterilizing effects;toothbrush
DO -
ER -
지윤정. (2012). Toothbrush sterilizing effects of using microwave. Journal of Korean society of Dental Hygiene, 12(3), 641-646.
지윤정. 2012, "Toothbrush sterilizing effects of using microwave", Journal of Korean society of Dental Hygiene, vol.12, no.3 pp.641-646. Available from: doi:
지윤정 "Toothbrush sterilizing effects of using microwave" Journal of Korean society of Dental Hygiene 12.3 pp.641-646 (2012) : 641.
지윤정. Toothbrush sterilizing effects of using microwave. 2012; 12(3), 641-646. Available from: doi:
지윤정. "Toothbrush sterilizing effects of using microwave" Journal of Korean society of Dental Hygiene 12, no.3 (2012) : 641-646.doi:
지윤정. Toothbrush sterilizing effects of using microwave. Journal of Korean society of Dental Hygiene, 12(3), 641-646. doi:
지윤정. Toothbrush sterilizing effects of using microwave. Journal of Korean society of Dental Hygiene. 2012; 12(3) 641-646. doi:
지윤정. Toothbrush sterilizing effects of using microwave. 2012; 12(3), 641-646. Available from: doi:
지윤정. "Toothbrush sterilizing effects of using microwave" Journal of Korean society of Dental Hygiene 12, no.3 (2012) : 641-646.doi: