Objectives: This study aimed to identify the levels of professional self-concept, empathy toward older adults, organizational commitment, and oral health care practices for older adults among dental hygienists and to examine related factors using hierarchical multiple regression. Methods: An online, self-administered survey was conducted from July 3 to July 30, 2025, among 210 dental hygienists with at least 3 years of clinical experience. Data were analyzed using descriptive statistics, independent t-tests, one-way ANOVA, Pearson’s correlation analysis, and hierarchical multiple regression. Results: Oral health care practices for older adults were positively correlated with professional self-concept (r=0.468, p<0.001), empathy toward older adults (r=0.490, p<0.001), and organizational commitment (r=0.312, p<0.001). In the final model, empathy toward older adults (β=0.367, p<0.001), professional self-concept (β=0.235, p=0.024), and clinical experience of 8 years or more (β=0.202, p=0.001) were significantly associated with oral health care practices for older adults, whereas organizational commitment was not significant. Conclusions: Empathy toward older adults and professional self-concept are key factors associated with oral health care practice for older adults. Educational programs focusing on empathy, professional role perception, and patient-centered practice for older adults are needed to strengthen dental hygienists’ competencies in oral health care for older adults.