Bacterial diversity in children&#039;s dental caries (소아의 치아 우식 부위별 세균 다양성)

김은미; Keunsik Baik (백근식); Ha,Myung-Ok (하명옥)

doi:10.13065/jksdh.2013.13.05.889

Bacterial diversity in children's dental caries

Journal of Korean society of Dental Hygiene
Abbr : J Korean Soc Dent Hyg
2013, 13(5), pp.889-900
DOI : 10.13065/jksdh.2013.13.05.889
Publisher : Korean Society of Dental Hygiene
Research Area : Medicine and Pharmacy > Dentistry

김은미 ¹, Keunsik Baik ², Ha,Myung-Ok ¹

¹광주보건대학교
²한국기초과학지원연구원

Accredited

ABSTRACT

Objectives : Molecular biology techniques were employed to assess diversity of bacterial in children's dental caries. Methods : DNA of germs was extracted and the diversity of the 16S rRNA clones was analyzed by amplified rDNA restriction analysis and sequencing. The experimental samples were pit and fissure caries (PC), deep dentinal caries (DC), smooth surface caries (SC), and supragingival plaque (PQ) from 50 children of age less than 12 years old. The control group was healthy teeth supragingival plaque (HT). Thirty clones from each 16S rRNA clone library of 5 samples were randomly selected,thus a total of 150 clones were analyzed. Results : Amplified rDNA restriction analysis uncovered 18, 20, 11, 17, and 22 phylotypes from healthy teeth, pit and fissure caries, deep dentinal caries, smooth surface caries, and supragingival plaque, respectively. Sequencing analysis found the dominance of Actinomycs naeslundii and Fusobacterium nucleatum in the healthy teeth; Leptotrichia sp. in the pit and fissure caries; Actinomyces sp., Streptococcus mutans, and Rahnella aquatilis in the deep dentinal caries;Streptococcus mutans and Actinomyces sp. in the smooth surface caries; Enterobacter hormaechei and Streptococcus sanguinis in the supragingival plaque. Conclusions : Clonal analysis identified 6 phyla, 20 genera, and 51 species.

KEYWORDS

ARDRA, bacterial diversity, dental caires, 16S rRNA gene

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Journal of Korean society of Dental Hygiene 2023 KCI Impact Factor : 0.8

Bacterial diversity in children's dental caries

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Citation status

* References for papers published after 2023 are currently being built.

Journal of Korean society of Dental Hygiene 2023 KCI Impact Factor : 0.8

Bacterial diversity in children's dental caries

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KEYWORDS

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