Apoptotic effect of Pseudomonas aeruginosa exotoxin A in human tongue squamous cell carcinoma(SCC) 25 cells (Pseudomonas aeruginosa exotoxin A (PEA)가 사람혀편평암종세포에서 나타나는 세포자멸사 작용)

최별보라; Gyoo-Cheon Kim (김규천)

doi:10.13065/jksdh.2014.14.04.601

Apoptotic effect of Pseudomonas aeruginosa exotoxin A in human tongue squamous cell carcinoma(SCC) 25 cells

Journal of Korean society of Dental Hygiene
Abbr : J Korean Soc Dent Hyg
2014, 14(4), pp.601-608
DOI : 10.13065/jksdh.2014.14.04.601
Publisher : Korean Society of Dental Hygiene
Research Area : Medicine and Pharmacy > Dentistry

최별보라 ¹, Gyoo-Cheon Kim ²

¹동서대학교
²부산대학교

Accredited

ABSTRACT

Objectives : The purpose of the study is to examine the apoptotic effects of Pseudomonas aeruginosa exotoxin A(PEA) in squamous cell carcinoma(SCC) 25 cells. Methods : Cell growth reduction and apoptosis induced by PEA were confirmed by WST-1 assay, Hoechst 33258 staining, flow cytometry analysis, and Western blot assay. Results : The PEA treatment decreased the cell viability in a dose and time dependent manner: control; 100±0e(p<0.01), 0.1875 nM; 87±4.36d(p<0.01), 0.375 nM; 82±0.58d(p<0.01), 0.75 nM; 72±1.67c(p<0.01), 1.5 nM; 51±1.53bc(p<0.01), 7.5 nM; 31±1.20ab(p<0.01), 15 nM; 26±0.67a(p<0.01), control; 100±0a(p<0.05), 24 h; 51±1.53b(p<0.05), 48 h; 16±0.5c(p<0.05), 72 h; 12±1.67d%(p<0.05). The PEA was observed on SCC 25 cells with the half maximal inhibitory concentration(IC50) value of 1.5 nM at 24 hours. The PEA treated SCC 25 cells demonstrated several types of apoptotic indications, such as nuclear condensation, the increase of sub G1, and the cleavage of PARP-1 and DFF 45. Conclusions : PEA showed anti-cancer activity against SCC 25 cells via apoptosis. PEA may potentially contribute to human oral cancer treatment.

KEYWORDS

apoptosis, Pseudomonas aeruginosa exotoxin A, squamous cell carcinoma

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Journal of Korean society of Dental Hygiene 2023 KCI Impact Factor : 0.8

Apoptotic effect of Pseudomonas aeruginosa exotoxin A in human tongue squamous cell carcinoma(SCC) 25 cells

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* References for papers published after 2023 are currently being built.

Journal of Korean society of Dental Hygiene 2023 KCI Impact Factor : 0.8

Apoptotic effect of Pseudomonas aeruginosa exotoxin A in human tongue squamous cell carcinoma(SCC) 25 cells

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