The effect of the cytotoxicity of sodium lauryl sulfate containing toothpaste on HaCaT and NIH-3T3 cells (구강세치제에 함유된 SLS(Sodium lauryl Sulfate)가 HaCaT 세포와 NIH-3T3 세포에 미치는 독성 효과)

PARK SANG RYE (박상례); Young Min Kim (김영민); choi byul bo ra (최별보라); Kim， Ji-young (김지영)

The effect of the cytotoxicity of sodium lauryl sulfate containing toothpaste on HaCaT and NIH-3T3 cells

Journal of Korean society of Dental Hygiene
Abbr : J Korean Soc Dent Hyg
2015, 15(4), pp.719-725
Publisher : Korean Society of Dental Hygiene
Research Area : Medicine and Pharmacy > Dentistry

PARK SANG RYE ¹, Young Min Kim ², choi byul bo ra ³, Kim， Ji-young ¹

¹경남정보대학교
²부산대학교
³동서대학교

Accredited

ABSTRACT

Objectives: The purpose of this study was to determine the toxic effects of sodium lauryl sulfate(SLS) in human keratinocyte HaCaT cells and mouse fibroblast NIH-3T3 cells. Methods: The effect of sodium lauryl sulfate(SLS) cell viability and proliferation were determined by WST-1 assay and changes shape of nucleus were evaluated by Hoechst staining under fluorescence microscopy. Additionally, observation of cell morphological changes under light microscopy. Results: SLS induced cytotoxicity and a marked apoptosis in both HaCaT and NIH-3T3 cell lines. With the result of the WST-1 assay, SLS induced the cytotoxicity of 0.005% and 0.0075%, 0.01% SLS for 24 h after HaCaT and NIH-3T3 cells in time and dose-dependent manner(p<0.005). SLS inhibited cell growth and caused apoptosis as evidenced by nuclear fragmentation and condensation. Thus, determination of the morphological changes to define apoptosis was visualized using inverted phase contrast microscopy. Conclusions: SLS had toxicity of the human keratinocyte cells and mouse fibroblast cells and this study will provide the basic data for the development of proper SLS concentration in dentifrice.

KEYWORDS

apoptosis, cytotoxicity, fibroblast, keratinocyte, sodium lauryl sulfate

Citation status

* References for papers published after 2023 are currently being built.

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