Comparison of quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction

Young-Sun Kim (김영선); Jung-Hwa Lee (이정화); Young-Eun Lee (이영은)

Comparison of quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction

Journal of Korean society of Dental Hygiene
Abbr : J Korean Soc Dent Hyg
2015, 15(6), pp.1063~1071
Publisher : Korean Society of Dental Hygiene
Research Area : Medicine and Pharmacy > Dentistry

Young-Sun Kim ¹, Jung-Hwa Lee ¹, Young-Eun Lee ¹

¹대구보건대학교

Accredited

ABSTRACT

Objectives: The purpose of the study is to investigate the quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction. Methods: Participants were voluntarily recruited at D university, and saliva samples were extracted before and after scaling. Multiple real-time polymerase chain reactions were used to analyze characteristics and the amount of nine kinds of periodontal pathogens; Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Parvimonas micra, Campylobacter rectus, and Eikenella corrodens. Results: After scaling, most periodontal pathogens except Eikenella corrodens were significantly decreased in all subjects(p<0.05). In addition, the percentage of microorganisms associated with disease, the microorganism risk index of periodontitis and the prevalence of red complex, orange complex, and Aggregatibacter actinomycetemcomitans was also significantly reduced after scaling(p<0.05). Conclusions: Scaling decreased in the amount of major periodontal pathogens and periodontitis prevalence rate.

KEYWORDS

periodontal pathogens, real-time polymerase chain reaction, scaling

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