Comparison of quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction

Kim, YoungSun (김영선); Lee Jung Hwa (이정화); LEE, YOUNGEUN (이영은)

Comparison of quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction

Journal of Korean society of Dental Hygiene
Abbr : J Korean Soc Dent Hyg
2015, 15(6), pp.1063-1071
Publisher : Korean Society of Dental Hygiene
Research Area : Medicine and Pharmacy > Dentistry

Kim, YoungSun ¹, Lee Jung Hwa ¹, LEE, YOUNGEUN ¹

¹대구보건대학교

Accredited

ABSTRACT

Objectives: The purpose of the study is to investigate the quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction. Methods: Participants were voluntarily recruited at D university, and saliva samples were extracted before and after scaling. Multiple real-time polymerase chain reactions were used to analyze characteristics and the amount of nine kinds of periodontal pathogens; Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Parvimonas micra, Campylobacter rectus, and Eikenella corrodens. Results: After scaling, most periodontal pathogens except Eikenella corrodens were significantly decreased in all subjects(p<0.05). In addition, the percentage of microorganisms associated with disease, the microorganism risk index of periodontitis and the prevalence of red complex, orange complex, and Aggregatibacter actinomycetemcomitans was also significantly reduced after scaling(p<0.05). Conclusions: Scaling decreased in the amount of major periodontal pathogens and periodontitis prevalence rate.

KEYWORDS

periodontal pathogens, real-time polymerase chain reaction, scaling

Citation status

* References for papers published after 2023 are currently being built.

[journal] 정유진 / 2015 / Influencing factors to dental caries and periodontal diseases in Korean adults / 한국치위생학회지 / 한국치위생학회 15(1) : 47~54

[journal] Mohangi GU / 2013 / Periodontal disease: mechanisms of infection and inflammation and possible impact on miscellaneous systemic diseases and conditions / SADJ 68 : 462~467

[journal] van Houte J / 1994 / Role of micro-organisms in caries etiology / J Dent Res 73 : 672~681

[journal] Petersen PE / 2005 / WHO's action for continuous improvement in oral health / Bull World Health Organ 83 : 642~

[journal] Lopez R / 2015 / On putative periodontal pathogens: an epidemiological perspective / Virulence 6 : 249~257

[journal] Theilade E / 1989 / Advances in oral microbiology / Ann R Australas Coll Dent Surg 10 : 62~71

[journal] Marchesan J / 2015 / TLR4, NOD1 and NOD2 mediate immune recognition of putative newly identified periodontal pathogens / Mol Oral Microbiol

[journal] Salminen A / 2015 / Quantitative PCR analysis of salivary pathogen burden in periodontitis / Front Cell Infect Microbiol 5 : 69~

[journal] Chahboun H / 2015 / Bacterial profile of aggressive periodontitis in Morocco: a cross-sectional study / BMC Oral Health 15 : 25~

[journal] Socransky SS / 1998 / Microbial complexes in subgingival plaque / J Clin Periodontol 25 : 134~144

[journal] Ximénez-Fyvie LA / 2000 / Microbial composition of supra- and subgingival plaque in subjects with adult periodontitis / J Clin Periodontol 27 : 722~732

[journal] Haubek D / 2004 / The highly leukotoxic JP2 clone of Actinobacillus actinomycetemcomitans and progression of periodontal attachment loss / J Dent Res 83 : 767~770

[journal] Arabaci T / 2007 / Sonic and ultrasonic scalers in periodontal treatment: a review / Int J Dent Hyg 5 : 2~12

[journal] Petersilka GJ / 2002 / Antimicrobial effects of mechanical debridement / Periodontol 2000 28 : 56~71

[journal] Darby I / 2009 / Non-surgical management of periodontal disease / Aust Dent J 54 : S86~S95

[journal] Cobb CM / 2002 / Clinical significance of non-surgical periodontal therapy: an evidence-based perspective of scaling and root planing / J Clin Periodontol 29 : 6~16

[journal] Faveri M / 2006 / Scaling and root planing and chlorhexidine mouthrinses in the treatment of chronic periodontitis: a randomized, placebo-controlled clinical trial / J Clin Periodontol 33 : 819~828

[journal] Heitz-Mayfield LJ / 2002 / A systematic review of the effect of surgical debridement vs non-surgical debridement for the treatment of chronic periodontitis / J Clin Periodontol 29 : 92~102

[journal] Haffajee AD / 1997 / Clinical and microbiological features of subjects with adult periodontitis who responded poorly to scaling and root planing / J Clin Periodontol 24 : 767~776

[journal] Knöfler GU / 2011 / Microbiologic findings 1 year after partialand full-mouth scaling in the treatment of moderate chronic periodontitis / Quintessence Int 42 : e107~e117

[journal] Yang H / 2008 / Association between Porphyromonas gingivalis and scaling and root planning therapy / Hua Xi Kou Qiang Yi Xue Za Zhi 26 : 147~151

[journal] Braga RR / 2010 / Quantification of five putative periodontal pathogens in female patients with and without chronic periodontitis by real-time polymerase chain reaction / Anaerobe 16 : 234~239

[journal] Salminen A / 2015 / Quantitative PCR analysis of salivary pathogen burden in periodontitis / Front Cell Infect Microbiol 5 : 69~

[journal] 김선미 / 2008 / Quantitative detection of periodontopathic bacteria using real-time PCR / 대한소아치과학회지 / 대한소아치과학회 35(3) : 494~503

[journal] Lee MR / 2011 / A study on the change of oral microorganism before and after oral hygiene management / Korea Academy of Mental Health Social Work 13 : 51~65

[journal] 조홍범 / 2013 / Multiplex Real-Time PCR for Simultaneous Detection of 6 Periodontopathic Bacteria / 미생물학회지 / 한국미생물학회 49(3) : 292~296

[book] Geneall Biotechnology / 2012 / DNA purification handbook / Geneall biotechnology : 11~38

[journal] Könönen E / 2007 / Population-based study of salivary carriage of periodontal pathogens in adults / J Clin Microbiol 45 : 2446~2451

[journal] 김미광 / 2004 / Comparison of Detection Rate of Prevotella intermedia and Prevotella nigrescens in Subgingival Plaque from Korean Periodontitis Patients / 대한구강보건학회지 / 대한예방치과·구강보건학회 28(2) : 279~284

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