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@article{ART002125492},
author={PARK SANG RYE and choi byul bo ra and Da-Hye Kim and 김지영},
title={The effects of sodium fluoride on oral normal cell cultured in vitro},
journal={Journal of Korean society of Dental Hygiene},
issn={2287-1705},
year={2016},
volume={16},
number={3},
pages={471-477},
doi={}

TY - JOUR
AU - PARK SANG RYE
AU - choi byul bo ra
AU - Da-Hye Kim
AU - 김지영
TI - The effects of sodium fluoride on oral normal cell cultured in vitro
JO - Journal of Korean society of Dental Hygiene
PY - 2016
VL - 16
IS - 3
PB - Korean Society of Dental Hygiene
SP - 471
EP - 477
SN - 2287-1705
AB - Objectives: Fluoride is widely used in the prevention and control of dental caries. The purpose of this study is to examine the biological effects of Sodium fluoride on the proliferation of oral normal cell in vitro(MDPC-23, HaCaT, HGF-1 cells). Methods: The proliferation of normal cells and the cyto-skeletal change of normal cells were assessed by WST-1 assay and F-actin stain assay. The statistical significances of the resulting data were analyzed using SPSS(Window 12.0). Results: The sodium fluoride(0-12 mM) treatment decreased the cell viability in a dose and time dependent manner: HaCaT(6 h): 100±0, 98±0.39, 82±2.68, 75±0.83, 69±1, 67±1.42%(p<0.005); HaCaT(24 h): 100±0, 98±1.85, 54±0.64, 43±0.4, 38±0.32, 36±0.13%(p<0.006), MDPC-23(6 h): 100±0, 93±1.48, 85±0.28, 82±1.58, 79±1.48, 76±1.93%(p<0.009); MDPC-23(24 h): 100±0, 91±1.26, 58±0.65, 49±1, 44±0.74, 2±0.05%(p<0.005), HGF-1(6 h): 100±0, 97±2.93, 89±5, 71±5.42, 58±4.82, 43±3.47%(p<0.009); HGF-1(24 h): 100±0, 97±2.05, 73±1.73, 22±1.61, 14±1.73, 7±0.85%(p<0.005). Thus, changes in cell morphology and disruption of filamentous(F)-actin organization were observed in higher concentration. Conclusions: These results suggest that higher concentrations of fluoride lead to a reduce the number of cells and morphology change of normal cell.
KW - human gingival fibroblast;keratinocyte;odontoblast;sodium fluoride
DO -
ER -

PARK SANG RYE, choi byul bo ra, Da-Hye Kim and 김지영. (2016). The effects of sodium fluoride on oral normal cell cultured in vitro. Journal of Korean society of Dental Hygiene, 16(3), 471-477.

PARK SANG RYE, choi byul bo ra, Da-Hye Kim and 김지영. 2016, "The effects of sodium fluoride on oral normal cell cultured in vitro", Journal of Korean society of Dental Hygiene, vol.16, no.3 pp.471-477. Available from: doi:

PARK SANG RYE, choi byul bo ra, Da-Hye Kim, 김지영 "The effects of sodium fluoride on oral normal cell cultured in vitro" Journal of Korean society of Dental Hygiene 16.3 pp.471-477 (2016) : 471.

PARK SANG RYE, choi byul bo ra, Da-Hye Kim, 김지영. The effects of sodium fluoride on oral normal cell cultured in vitro. 2016; 16(3), 471-477. Available from: doi:

PARK SANG RYE, choi byul bo ra, Da-Hye Kim and 김지영. "The effects of sodium fluoride on oral normal cell cultured in vitro" Journal of Korean society of Dental Hygiene 16, no.3 (2016) : 471-477.doi:

PARK SANG RYE; choi byul bo ra; Da-Hye Kim; 김지영. The effects of sodium fluoride on oral normal cell cultured in vitro. Journal of Korean society of Dental Hygiene, 16(3), 471-477. doi:

PARK SANG RYE; choi byul bo ra; Da-Hye Kim; 김지영. The effects of sodium fluoride on oral normal cell cultured in vitro. Journal of Korean society of Dental Hygiene. 2016; 16(3) 471-477. doi:

PARK SANG RYE, choi byul bo ra, Da-Hye Kim, 김지영. The effects of sodium fluoride on oral normal cell cultured in vitro. 2016; 16(3), 471-477. Available from: doi:

PARK SANG RYE, choi byul bo ra, Da-Hye Kim and 김지영. "The effects of sodium fluoride on oral normal cell cultured in vitro" Journal of Korean society of Dental Hygiene 16, no.3 (2016) : 471-477.doi: