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@article{ART002768229},
author={So-Yeong Park},
title={Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells},
journal={Journal of Korean society of Dental Hygiene},
issn={2287-1705},
year={2021},
volume={21},
number={5},
pages={545-553},
doi={10.13065/jksdh.20210052}

TY - JOUR
AU - So-Yeong Park
TI - Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells
JO - Journal of Korean society of Dental Hygiene
PY - 2021
VL - 21
IS - 5
PB - Korean Society of Dental Hygiene
SP - 545
EP - 553
SN - 2287-1705
AB - Objectives: This study aimed to investigate the cytotoxicity of Nexus RMGIC, an indirect restorative cement, on cell survival rate and reactive oxygen species (ROS) production in periodontal stem cells (PDSCs). Methods: PDSCs were incubated with serially diluted Nexus RMGIC eluates with and without the addition of N-acetyl-cysteine (NAC). Cell survival was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ROS generation was determined by measuring the fluorescence intensity for 2′,7′-dichlorofluorescin diacetate. Results: Nexus RMGIC exposure decreased cell proliferation and cell survival rate in a dose-dependent manner (1:8, 1:4, 1:2, 1:1) in PDSCs. The cytotoxicity of Nexus RMGIC was inhibited by treatment with 10-mM NAC. In addition, the production of ROS was detected by immunofluorescence after PDSCs were exposed to Nexus RMGIC. However, ROS generation was significantly suppressed in the NAC pretreatment compared with the Nexus RMGIC group. Conclusions: Nexus RMGIC increased the cytotoxicity and ROS generation. ROS was involved in Nexus RMGIC-induced cell toxicity.
KW - Acrylic resin;Cytotoxicity;Dental cement;N-acetyl-L-cysteine;Reactive oxygen species
DO - 10.13065/jksdh.20210052
ER -

So-Yeong Park. (2021). Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells. Journal of Korean society of Dental Hygiene, 21(5), 545-553.

So-Yeong Park. 2021, "Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells", Journal of Korean society of Dental Hygiene, vol.21, no.5 pp.545-553. Available from: doi:10.13065/jksdh.20210052

So-Yeong Park "Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells" Journal of Korean society of Dental Hygiene 21.5 pp.545-553 (2021) : 545.

So-Yeong Park. Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells. 2021; 21(5), 545-553. Available from: doi:10.13065/jksdh.20210052

So-Yeong Park. "Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells" Journal of Korean society of Dental Hygiene 21, no.5 (2021) : 545-553.doi: 10.13065/jksdh.20210052

So-Yeong Park. Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells. Journal of Korean society of Dental Hygiene, 21(5), 545-553. doi: 10.13065/jksdh.20210052

So-Yeong Park. Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells. Journal of Korean society of Dental Hygiene. 2021; 21(5) 545-553. doi: 10.13065/jksdh.20210052

So-Yeong Park. Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells. 2021; 21(5), 545-553. Available from: doi:10.13065/jksdh.20210052

So-Yeong Park. "Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells" Journal of Korean society of Dental Hygiene 21, no.5 (2021) : 545-553.doi: 10.13065/jksdh.20210052